Staphylococcus aureus dry-surface biofilms are not killed by sodium hypochlorite: implications for infection control

A Almatroudi; I B Gosbell; H Hu; S O Jensen; B A Espedido; S Tahir; T O Glasbey; P Legge; G Whiteley; A Deva; K Vickery

doi:10.1016/j.jhin.2016.03.020

Staphylococcus aureus dry-surface biofilms are not killed by sodium hypochlorite: implications for infection control

J Hosp Infect. 2016 Jul;93(3):263-70. doi: 10.1016/j.jhin.2016.03.020. Epub 2016 Apr 12.

Authors

A Almatroudi¹, I B Gosbell², H Hu³, S O Jensen⁴, B A Espedido⁴, S Tahir³, T O Glasbey⁵, P Legge³, G Whiteley⁵, A Deva³, K Vickery⁶

Affiliations

¹ Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, New South Wales, Australia; Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Qassim, Saudi Arabia.
² Antibiotic Resistance and Mobile Elements Group, Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia; Molecular Medicine Research Group, School of Medicine, Western Sydney University, New South Wales, Australia; Department of Microbiology & Infectious Diseases, Sydney South West Pathology Service - Liverpool, New South Wales Health Pathology, New South Wales, Australia.
³ Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, New South Wales, Australia.
⁴ Antibiotic Resistance and Mobile Elements Group, Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia; Molecular Medicine Research Group, School of Medicine, Western Sydney University, New South Wales, Australia.
⁵ Whiteley Corporation, North Sydney, New South Wales, Australia.
⁶ Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, New South Wales, Australia. Electronic address: Karen.vickery@mq.edu.au.

PMID: 27140421
DOI: 10.1016/j.jhin.2016.03.020

Abstract

Background: Dry hospital environments are contaminated with pathogenic bacteria in biofilms, which suggests that current cleaning practices and disinfectants are failing.

Aim: To test the efficacy of sodium hypochlorite solution against Staphylococcus aureus dry-surface biofilms.

Methods: The Centers for Disease Control and Prevention Biofilm Reactor was adapted to create a dry-surface biofilm, containing 1.36 × 10(7)S. aureus/coupon, by alternating cycles of growth and dehydration over 12 days. Biofilm was detected qualitatively using live/dead stain confocal laser scanning microscopy (CLSM), and quantitatively with sonicated viable plate counts and crystal violet assay. Sodium hypochlorite (1000-20,000parts per million) was applied to the dry-surface biofilm for 10min, coupons were rinsed three times, and residual biofilm viability was determined by CLSM, plate counts and prolonged culture up to 16 days. Isolates before and after exposure underwent minimum inhibitory concentration (MIC) and minimum eradication concentration (MEC) testing, and one pair underwent whole-genome sequencing.

Findings: Hypochlorite exposure reduced plate counts by a factor of 7 log10, and reduced biofilm biomass by a factor of 100; however, staining of residual biofilm showed that live S. aureus cells remained. On prolonged incubation, S. aureus regrew and formed biofilms. Post-exposure S. aureus isolates had MICs and MECs that were not significantly different from the parent strains. Whole-genome sequencing of one pre- and post-exposure pair found that they were virtually identical.

Conclusions: Hypochlorite exposure led to a 7-log kill but the organisms regrew. No resistance mutations occurred, implying that hypochlorite resistance is an intrinsic property of S. aureus biofilms. The clinical significance of this warrants further study.

Keywords: Biofilms; Disinfectants; Dry-surface biofilms; Healthcare-associated infections; Hospital cleaning; Infection control; Sodium hypochlorite; Staphylococcus aureus.

MeSH terms

Biofilms / drug effects*
Colony Count, Microbial
Dehydration
Disinfectants / pharmacology*
Environmental Microbiology
Infection Control / methods
Microbial Sensitivity Tests
Microbial Viability / drug effects*
Microscopy, Confocal
Sodium Hypochlorite / pharmacology*
Staphylococcus aureus / drug effects*
Staphylococcus aureus / physiology*
Surface Properties*

Substances

Disinfectants
Sodium Hypochlorite