The mechanism of action of FXR1P-related miR-19b-3p in SH-SY5Y

Gene. 2016 Aug 15;588(1):62-8. doi: 10.1016/j.gene.2016.04.037. Epub 2016 Apr 29.

Abstract

The biological effects of microRNAs (miRNAs) in the Fragile X Syndrome (FXS) have been widely studied. Dysregulation of miRNAs plays a critical role in the progression of nervous system diseases and in cell proliferation and differentiation. Our previous study validated that miR-19b-3p was associated with FXR1 (Fragile X related gene 1), one of homologous genes of FMR1 (Fragile X mental retardation 1). The purpose of this study was to investigate the relationship of FXR1 and miR-19b-3p, and the crucial role of miR-19b-3p in FXS and to validate whether miR-19b-3p could regulate the growth of SH-SY5Y cells. We determined that miR-19b-3p could regulate the expression of not only USP32, RAB18 and Dusp6 but also FXR1, and FXR1 could in turn regulate the expression of miR-19b-3p. What's more, the overexpression of miR-19b-3p significantly inhibited the proliferation, contributed the apoptosis and slowed down the cycle of SH-SY5Y cells. Taken together, our results indicate that miR-19b-3p plays a significant role in the molecular pathology of FXS by interacting with FXR1 and influencing the growth of SH-SY5Y cells.

Keywords: Dusp6; FXR1; RAB18; USP32; miR-19b.

MeSH terms

  • Apoptosis
  • Autistic Disorder / genetics
  • Cell Cycle
  • Cell Line
  • Cell Proliferation
  • Dual Specificity Phosphatase 6 / genetics
  • Gene Expression Regulation*
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism*
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism*
  • Ubiquitin Thiolesterase / genetics
  • rab GTP-Binding Proteins / genetics

Substances

  • FXR1 protein, human
  • MIRN19 microRNA, human
  • MicroRNAs
  • RAB18 protein, human
  • RNA-Binding Proteins
  • USP10 protein, human
  • Dual Specificity Phosphatase 6
  • Ubiquitin Thiolesterase
  • rab GTP-Binding Proteins