Abstract
In this study, we evaluated the immune responses of avian metapneumovirus harboring chicken Fc molecule. Stable Vero cells expressing chicken Fc chimera on its surface (Vero-cFc) were established, and we confirmed that aMPV grown in Vero-cFc incorporated host derived chimera Fc into the aMPV virions. Immunization of chicken with aMPV-cFc induced higher level of antibodies and inflammatory cytokines; (Interferon (IFN)-γ and Interleukin (IL)-1β) compared to those of aMPV. The increased levels of antibodies and inflammatory cytokines in chicken immunized with aMPV-cFc were statistically significantly (p<0.05) to that of aMPV and control. The aMPV-cFc group also generated the highest neutralizing antibody response. After challenges, chickens immunized with aMPV-cFc showed much less pathological signs in nasal turbinates and trachea so that we could confirm aMPV-cFc induced higher protection than that of aMPV. The greater ability of aMPV harboring chicken Fc to that of aMPV presented it as a possible vaccine candidate.
Keywords:
Antibody response; Avian metapneumovirus (aMPV); Chicken Fc; Cytokines; Neutralizing antibodies; Vaccines.
Copyright © 2016 Elsevier B.V. All rights reserved.
MeSH terms
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Animals
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Antibodies, Neutralizing / biosynthesis*
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Antibodies, Viral / biosynthesis*
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Chickens / immunology*
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Chickens / virology
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Chlorocebus aethiops
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Gene Expression
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Immunization
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Immunoglobulin Fc Fragments / genetics*
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Interferon-gamma / biosynthesis
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Interferon-gamma / immunology
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Interleukin-1beta / biosynthesis
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Interleukin-1beta / immunology
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Metapneumovirus / drug effects
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Metapneumovirus / growth & development
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Metapneumovirus / immunology
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Paramyxoviridae Infections / immunology
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Paramyxoviridae Infections / prevention & control
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Paramyxoviridae Infections / veterinary*
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Paramyxoviridae Infections / virology
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Plasmids / chemistry
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Plasmids / immunology
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Poultry Diseases / immunology
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Poultry Diseases / prevention & control*
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Poultry Diseases / virology
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Recombinant Fusion Proteins / administration & dosage
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Vero Cells
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Viral Proteins / genetics
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Viral Proteins / immunology
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Viral Vaccines / administration & dosage*
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Viral Vaccines / genetics
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Viral Vaccines / immunology
Substances
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Antibodies, Neutralizing
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Antibodies, Viral
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Immunoglobulin Fc Fragments
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Interleukin-1beta
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Recombinant Fusion Proteins
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Viral Proteins
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Viral Vaccines
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Interferon-gamma