A "turn-on" fluorescent chemosensor for the detection of Zn(II) in aqueous solution at neutral pH and its application in live cells imaging

Yuanyuan Li; Kai Li; Juan He

doi:10.1016/j.talanta.2016.03.040

A "turn-on" fluorescent chemosensor for the detection of Zn(II) in aqueous solution at neutral pH and its application in live cells imaging

Talanta. 2016 Jun 1:153:381-5. doi: 10.1016/j.talanta.2016.03.040. Epub 2016 Mar 13.

Authors

Yuanyuan Li¹, Kai Li², Juan He¹

Affiliations

¹ School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, PR China.
² College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China. Electronic address: likai@zzu.edu.cn.

PMID: 27130131
DOI: 10.1016/j.talanta.2016.03.040

Abstract

Pyridoxal-2-hydrazinopyridine Schiff-base (1) was prepared by one-step synthesis from commercially available materials of pyridoxal and 2-hydrazinopyridine. Compound 1 could be applied as a fluorescent chemosensor for Zn(II) in aqueous solution at pH 7.4. After binding to Zn(II) with a molar ratio of 1:1, 1 exhibited 17-fold fluorescence enhancement. In addition, 1 showed a good selectivity toward Zn(II) over other metal ions, which can especially distinguish Zn(II) from Cd(II). A linear range of 5.0-20.0μmolL(-1) and a detection limit of 70nmolL(-1) were also achieved. Moreover, the utility of chemosensor 1 in bioimaging of Zn(II) in living cells was demonstrated, which suggested that 1 could be used as a Zn(II)-selective chemosensor in biological samples.

Keywords: Aqueous solution; Cell imaging; Fluorescent chemosensor; Zinc; “Turn on” response.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Survival
Fluorescent Dyes
Hydrogen-Ion Concentration
Pyridones
Solutions
Spectrometry, Fluorescence*
Zinc

Substances

Fluorescent Dyes
Pyridones
Solutions
2-hydrazinopyridine
Zinc