Benzochloroporphyrin derivative photosensitizer-mediated photodynamic therapy for Ewing sarcoma

Mengxiong Sun; Chenghao Zhou; Hui Zeng; Fei Yin; Zhuoying Wang; Jianzhong Yao; Yingqi Hua; Zhengdong Cai

doi:10.1016/j.jphotobiol.2016.03.059

Benzochloroporphyrin derivative photosensitizer-mediated photodynamic therapy for Ewing sarcoma

J Photochem Photobiol B. 2016 Jul:160:178-84. doi: 10.1016/j.jphotobiol.2016.03.059. Epub 2016 Apr 8.

Authors

Mengxiong Sun¹, Chenghao Zhou¹, Hui Zeng², Fei Yin³, Zhuoying Wang¹, Jianzhong Yao⁴, Yingqi Hua⁵, Zhengdong Cai⁶

Affiliations

¹ Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
² Department of Orthopedics, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, China.
³ The Advanced Institute of Translational Medicine, Tongji University, Shanghai 200092, China.
⁴ School of Pharmacy, Second Military Medical University, Shanghai 200433, China. Electronic address: yaojz@sh163.net.
⁵ Department of Orthopedics, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, China; The Advanced Institute of Translational Medicine, Tongji University, Shanghai 200092, China. Electronic address: yingqi.hua@yahoo.com.
⁶ Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China; Department of Orthopedics, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, China. Electronic address: zhdcai@yeah.net.

PMID: 27113445
DOI: 10.1016/j.jphotobiol.2016.03.059

Abstract

In this study, we evaluated the photodynamic efficacy of a new photosensitizer, benzochloroporphyrin derivative 18 (BCPD-18), in Ewing sarcoma. We found that BCPD-18 decreased the viability of TC-71 cells irradiated by 670nm laser in a concentration dependent manner. We also observed cells undergoing apoptosis as well as cell cycle arrest at the G2M phase after BCPD-18-mediated photodynamic therapy (BCPD-PDT). In addition, in vivo study (subcutaneous and orthotopic models) showed that BCPD-PDT reduced tumor size, tumor weight and tumor-bearing leg weight. After PDT, apoptosis was shown in vivo. Bax expression was increased, and Bcl-2 expression was decreased. This study provides evidence that BCPD-18 could probably be a useful photosensitizer in PDT for Ewing sarcoma.

Keywords: Benzochloroporphyrin derivatives; Ewing sarcoma; Photodynamic therapy.

MeSH terms

Animals
Apoptosis / drug effects*
Bone Neoplasms / drug therapy
Bone Neoplasms / metabolism
Bone Neoplasms / pathology
Cell Line, Tumor
Disease Models, Animal
G2 Phase Cell Cycle Checkpoints / drug effects
Humans
Mice
Mice, Nude
Photochemotherapy
Photosensitizing Agents / chemistry
Photosensitizing Agents / therapeutic use
Photosensitizing Agents / toxicity*
Porphyrins / chemistry
Porphyrins / therapeutic use
Porphyrins / toxicity*
Proto-Oncogene Proteins c-bcl-2 / metabolism
Sarcoma, Ewing / drug therapy
Sarcoma, Ewing / metabolism
Sarcoma, Ewing / pathology
Transplantation, Heterologous
bcl-2-Associated X Protein / metabolism

Substances

Photosensitizing Agents
Porphyrins
Proto-Oncogene Proteins c-bcl-2
bcl-2-Associated X Protein