Fontibacillus pullulanilyticus sp.nov. isolated from soil

Abstract

Gram stain-negative, motile, catalase-, and oxidase- positive strain, designated DSHK107(T) was isolated from soil of Cukurova University campus in Adana, Turkey. Its taxonomy was investigated using a polyphasic approach. The strain grew at 20-42 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 7.0) and in 0-2.0% NaCl (w/v). 16S rRNA gene sequence analysis revealed that the strain belonged to the genus Fontibacillus; strain DSHK107(T) showed highest sequence similarity to type strains Fontibacillus aquaticus GPTSA 19(T) (97.8%) and Fontibacillus panacisegetis P11-6(T) (97.0%). The major fatty acid of strain DSHK107(T) was anteiso-C15:0 (46.7). The polar lipids of strain DSHK107(T) consisted of dihosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unknown phospholipids, four unknown lipids, three unknown glycolipids, two unknown aminophospholipids, an unknown aminolipid, and an unknown aminophosphoglycolipid. The major isoprenoid quinone was MK-7. The DNA G+C content of DSHK107(T) was 42.1 mol%. DNA-DNA hybridization showed that the strain DSHK107(T) shared low DNA-DNA relatedness with F. aquaticus DSM 17643(T) , Fontibacillus solani A4STRO4(T) , and F. panacisegetis DSM 28129(T) (47, 58, and 59.3%, respectively). Thus, our results support the placement of strain DSHK107(T) within a separate and previously unrecognized species. On the basis of a taxonomic study using a polyphasic approach, strain DSHK107(T) is considered to represent a novel species of the genus Fontibacillus, for which the name Fontibacillus pullulanilyticus sp. nov. is proposed. The type strain is DSHK107(T) (=NCCB 100560(T) = DSM 100116(T) ).

Keywords: Fontibacillus pullulanilyticus; Paenibacillaceae; Polyphasic taxonomy.