Proteomic Profile of Brucella abortus-Infected Bovine Chorioallantoic Membrane Explants

PLoS One. 2016 Apr 22;11(4):e0154209. doi: 10.1371/journal.pone.0154209. eCollection 2016.

Abstract

Brucella abortus is the etiological agent of bovine brucellosis, a zoonotic disease that causes significant economic losses worldwide. The differential proteomic profile of bovine chorioallantoic membrane (CAM) explants at early stages of infection with B. abortus (0.5, 2, 4, and 8 h) was determined. Analysis of CAM explants at 0.5 and 4 h showed the highest differences between uninfected and infected CAM explants, and therefore were used for the Differential Gel Electrophoresis (DIGE). A total of 103 spots were present in only one experimental group and were selected for identification by mass spectrometry (MALDI/ToF-ToF). Proteins only identified in extracts of CAM explants infected with B. abortus were related to recognition of PAMPs by TLR, production of reactive oxygen species, intracellular trafficking, and inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brucella abortus / physiology
  • Brucellosis, Bovine / metabolism*
  • Brucellosis, Bovine / microbiology
  • Cattle
  • Chorioallantoic Membrane / metabolism*
  • Chorioallantoic Membrane / microbiology
  • Culture Techniques
  • Electrophoresis, Gel, Two-Dimensional
  • Female
  • Host-Pathogen Interactions
  • Mass Spectrometry
  • Proteome / metabolism*
  • Proteomics / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Trophoblasts / cytology
  • Trophoblasts / metabolism
  • Trophoblasts / microbiology

Substances

  • Proteome

Grants and funding

This study was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and the Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG – PRONEX). JPSM, APL, and RLS have fellowships from CNPq.