Objective: This study investigated the osteoblastic behavior to yttrium-stabilized tetragonal zirconia polycrystal (Y-TZP) surface after different intensities of erbium, chromium-doped:yttrium, scandium, gallium, and garnet (Er,Cr:YSGG) laser irradiation treatment.
Materials and methods: Y-TZP blocks were randomized divided into 4 groups to Er,Cr:YSGG laser irradiation: group G1 = control (no laser irradiation); group G2 = 1.5 W; group G3 = 3.0 W; group G4 = 5.0 W, with air-water irrigation. The irradiated surface roughness (Sa and Ra) topography was evaluated. MC3T3-E1 osteoblast cells were cultured and seeded onto the treated surfaces at a plate density of 1 × 10 cells/mL. At the third and seventh days cell proliferation was measured by the colorimetric assay methods. The specimens were prepared to scanning electron microscope to observe adhesion and morphology of cells.
Results: MTT assay showed adhesion and proliferation of MC3T3-E1 cells in all groups. Compared with group G1 (control), the group G2 had a proliferation 1.4% higher (P > 0.05), the group G3 had a proliferation 3.1% higher (P < 0.05) and group G4 had a proliferation 4.5% higher (P < 0.01). After 7 days there was no difference among the groups (ANOVA, Newman-Keuls).
Conclusions: Er,Cr:YSGG laser irradiation may have contributed to the greater and earlier cell spreading that was observed with the Y-TZP without alterations on surface topography.