The roles of selenium, insulin-like growth factor binding protein 2 and suppressor of cytokine signaling 3 in the pathogenesis of Kashin-Beck disease

Sen Wang; Chen Duan; Huan Liu; Wanzhen Shao; Cuiyan Wu; Jing Han; Xiong Guo

doi:10.3109/1354750X.2016.1141990

The roles of selenium, insulin-like growth factor binding protein 2 and suppressor of cytokine signaling 3 in the pathogenesis of Kashin-Beck disease

Biomarkers. 2016 Jul;21(5):409-15. doi: 10.3109/1354750X.2016.1141990. Epub 2016 Apr 21.

Authors

Sen Wang^{1

2}, Chen Duan^{1

2}, Huan Liu^{1

2}, Wanzhen Shao^{1

2}, Cuiyan Wu^{1

2}, Jing Han¹, Xiong Guo^{1

2}

Affiliations

¹ a School of Public Health, Health Science Center of Xi'an Jiaotong University , Xi'an , PR China and.
² b Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission , Xi'an , PR China.

PMID: 27099071
DOI: 10.3109/1354750X.2016.1141990

Abstract

We aimed to verify the levels of IGFBP2 and SOCS3 in cartilage and chondrocytes of Kashin-Beck disease (KBD) patients and the effects of different selenium concentrations on the protein expression levels. Chondrocytes were cultured with sodium selenite in vitro. Immunohistochemistry and western blotting were used to verify the protein expressions. IGFBP2 and SOCS3 were up-regulated in KBD chondrocytes and decreased with increasing selenium concentrations. IGFBP2 expressed highest in the middle zone of KBD cartilage, SOCS3 expressed higher in the middle and deep zone. IGFBP2 and SOCS3 may be the biomarkers for KBD diagnosis and evaluating the effect of selenium supplement.

Keywords: Biomarker; IGF-1 signaling pathway; IGFBP2; SOCS3; chondrocyte; osteoarthropathy.

MeSH terms

Biomarkers, Pharmacological / analysis
Cartilage, Articular / metabolism
Cartilage, Articular / pathology
Cells, Cultured
Chondrocytes / metabolism
Chondrocytes / pathology
Gene Expression Regulation / drug effects
Humans
Insulin-Like Growth Factor Binding Protein 2 / analysis
Insulin-Like Growth Factor Binding Protein 2 / physiology*
Kashin-Beck Disease / drug therapy
Kashin-Beck Disease / etiology
Kashin-Beck Disease / pathology*
Selenium / pharmacology*
Selenium / therapeutic use
Suppressor of Cytokine Signaling 3 Protein / analysis
Suppressor of Cytokine Signaling 3 Protein / physiology*

Substances

Biomarkers, Pharmacological
Insulin-Like Growth Factor Binding Protein 2
Suppressor of Cytokine Signaling 3 Protein
Selenium