Abstract
The intestinal surface is directly exposed to both commensal microorganisms as well as pathogens with a single layer of epithelium separating luminal microorganisms from internal tissues. Antimicrobial peptides play a crucial role in allowing epithelial cells to contain in the lumen beneficial and pathogenic microorganisms. The commensal dependent, epithelial produced, Th2 cytokine IL-25 can induce IL-13 and potentially the antimicrobial peptide angiogenin-4. Here we show that IL-13 downstream of IL-25 is required to induce angiogenin-4. IL-25 mediated induction of angiogenin-4 is furthermore not dependent on IL-22 or IL-17.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Blotting, Western
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Cells, Cultured
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Epithelial Cells / cytology
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Epithelial Cells / drug effects
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Epithelial Cells / metabolism*
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Gene Expression Regulation / drug effects*
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Immunoenzyme Techniques
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Interleukin-13 / genetics
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Interleukin-13 / metabolism*
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Interleukins / pharmacology*
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Intestinal Mucosa / cytology
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Intestinal Mucosa / drug effects
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Intestinal Mucosa / metabolism*
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Male
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Mice
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Mice, Inbred CBA
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RNA, Messenger / genetics
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Real-Time Polymerase Chain Reaction
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Reverse Transcriptase Polymerase Chain Reaction
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Ribonuclease, Pancreatic / genetics
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Ribonuclease, Pancreatic / metabolism*
Substances
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Interleukin-13
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Interleukins
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Mydgf protein, mouse
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RNA, Messenger
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Ang4 protein, mouse
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Ribonuclease, Pancreatic