Lensless two-photon imaging through a multicore fiber with coherence-gated digital phase conjugation

J Biomed Opt. 2016 Apr 30;21(4):45002. doi: 10.1117/1.JBO.21.4.045002.

Abstract

We performed near-diffraction limited two-photon fluorescence (TPF) imaging through a lensless, multicore-fiber (MCF) endoscope utilizing digital phase conjugation. The phase conjugation technique is compatible with commercially available MCFs with high core density. We demonstrate focusing of ultrashort pulses through an MCF and show that the method allows for resolution that is not limited by the MCF core spacing. We constructed TPF images of fluorescent beads and cells by digital scanning of the phase-conjugated focus on the target object and collection of the emitted fluorescence through the MCF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Endoscopes
  • Fiber Optic Technology / instrumentation
  • Fluorescent Dyes
  • HCT116 Cells
  • Holography / methods*
  • Humans
  • Image Processing, Computer-Assisted / methods*
  • Optical Imaging / instrumentation*
  • Optical Imaging / methods*

Substances

  • Fluorescent Dyes