Studies of silicon nanoparticles uptake and biodegradation in cancer cells by Raman spectroscopy

Elen Tolstik; Liubov Andreevna Osminkina; Christian Matthäus; Markus Burkhardt; Konstantine Eduardovich Tsurikov; Uliana Alexandrovna Natashina; Victor Yurievich Timoshenko; Rainer Heintzmann; Juergen Popp; Vladimir Sivakov

doi:10.1016/j.nano.2016.04.004

Studies of silicon nanoparticles uptake and biodegradation in cancer cells by Raman spectroscopy

Nanomedicine. 2016 Oct;12(7):1931-1940. doi: 10.1016/j.nano.2016.04.004. Epub 2016 Apr 13.

Affiliations

¹ Leibniz Institute of Photonic Technology, Jena, Germany; Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller-University, Jena Germany. Electronic address: Elen.tolstik@ipht-jena.de.
² Lomonosov Moscow State University, Physics Department, Moscow, Russia; National Research Nuclear University MEPhI, Moscow, Russia.
³ Leibniz Institute of Photonic Technology, Jena, Germany; Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller-University, Jena Germany.
⁴ Leibniz Institute of Photonic Technology, Jena, Germany.
⁵ Lomonosov Moscow State University, Physics Department, Moscow, Russia.

PMID: 27085902
DOI: 10.1016/j.nano.2016.04.004

Abstract

In-vitro Raman micro-spectroscopy was used for diagnostics of the processes of uptake and biodegradation of porous silicon nanoparticles (SiNPs) in breast cancer cells (MCF-7 cell line). Two types of nanoparticles, with and without photoluminescence in the visible spectral range, were investigated. The spatial distribution of photoluminescent SiNPs within the cells obtained by Raman imaging was verified by high-resolution structured-illumination optical microscopy. Nearly complete biodegradation of SiNPs inside the living cells was observed after 13days of the incubation. The results reveal new prospects of multi-modal visualization of SiNPs inside cancer cells for theranostic applications.

Keywords: Biodegradation; Cancer theranostic; HR-SIM microscopy; Raman spectroscopy; Silicon nanoparticles.

MeSH terms

Humans
MCF-7 Cells
Nanoparticles*
Optical Imaging / methods
Porosity
Silicon / pharmacokinetics*
Silicon Dioxide
Spectrum Analysis, Raman

Substances

Silicon Dioxide
Silicon