Identification of Wohlfahrtiimonas chitiniclastica isolated from an infected cow with hoof fetlow, China

Infect Genet Evol. 2016 Jul:41:174-176. doi: 10.1016/j.meegid.2016.04.008. Epub 2016 Apr 11.

Abstract

Wohlfahrtiimonas chitiniclastica is a rare but an emerging zoonotic pathogen. Here, we report the isolation and identification of W. chitiniclastica strain DZ2015 from hoof pus of an infected cow with hoof fetlow in Shandong, China by 16S rRNA gene sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Antimicrobial susceptibility testing and mouse infection experiments showed that the strain of W. chitiniclastica had broad susceptibility and was pathogenic to mice. This is the first report of the W. chitiniclastica isolated from an infected domestic animal in China.

Keywords: Antimicrobial susceptibility testing; Cow; Identification; Pathogenicity; Wohlfahrtiimonas chitiniclastica.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Cattle
  • Cattle Diseases / diagnosis
  • Cattle Diseases / drug therapy
  • Cattle Diseases / microbiology*
  • China
  • DNA, Bacterial / genetics*
  • Female
  • Gammaproteobacteria / drug effects
  • Gammaproteobacteria / genetics*
  • Gammaproteobacteria / isolation & purification
  • Gram-Negative Bacterial Infections / diagnosis
  • Gram-Negative Bacterial Infections / drug therapy
  • Gram-Negative Bacterial Infections / microbiology
  • Gram-Negative Bacterial Infections / veterinary*
  • Hoof and Claw / microbiology
  • Mice, Inbred BALB C
  • Microbial Sensitivity Tests
  • RNA, Ribosomal, 16S / genetics*
  • Sequence Analysis, DNA
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Suppuration / diagnosis
  • Suppuration / drug therapy
  • Suppuration / microbiology
  • Suppuration / veterinary*

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • RNA, Ribosomal, 16S