Structure of the O-specific polysaccharide from the marine bacterium Rheinheimera japonica KMM 9513(T), containing N-glycosidic bond between monosaccharides

Maxim S Kokoulin; Anatoly I Kalinovsky; Svetlana V Tomshich; Lyudmila A Romanenko; Valery V Mikhailov; Nadezhda A Komandrova

doi:10.1016/j.carres.2016.03.027

Structure of the O-specific polysaccharide from the marine bacterium Rheinheimera japonica KMM 9513(T), containing N-glycosidic bond between monosaccharides

Carbohydr Res. 2016 Jun 2:427:6-12. doi: 10.1016/j.carres.2016.03.027. Epub 2016 Apr 6.

Authors

Maxim S Kokoulin¹, Anatoly I Kalinovsky², Svetlana V Tomshich², Lyudmila A Romanenko², Valery V Mikhailov³, Nadezhda A Komandrova²

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russia. Electronic address: maxchem@mail.ru.
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russia.
³ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russia; Far Eastern Federal University, Suhanova St., 8, 690950 Vladivostok, Russia.

PMID: 27077821
DOI: 10.1016/j.carres.2016.03.027

Abstract

The O-specific polysaccharide was isolated from the lipopolysaccharide of type strain Rheinheimera japonica KMM 9513(T) and studied by sugar analysis, Smith degradation, and two-dimensional (1)H and (13)C NMR spectroscopy including (1)H,(1)H-TOCSY, (1)H,(1)H-COSY, (1)H,(1)H-ROESY, (1)H,(13)C-HSQC, (1)H,(13)C-HMBC, (1)H,(13)C-H2BC and (1)H,(13)C-HSQC-TOCSY experiments. The new structure of the O-specific polysaccharide of R. japonica KMM 9513(T) containing N-glycosidic bond was established.

Keywords: Marine bacterium; N-Glycosidic bond; O-Specific polysaccharide; Rheinheimera japonica; Structure.

MeSH terms

Carbohydrate Sequence
Chromatiaceae / chemistry
Chromatiaceae / metabolism*
Glycosides / chemistry
Magnetic Resonance Spectroscopy
Molecular Structure
Monosaccharides / chemistry
O Antigens / chemistry*

Substances

Glycosides
Monosaccharides
O Antigens