Immunoproteomic Approach for Screening Vaccine Candidates from Bacterial Outer Membrane Proteins

Jianyi Pan; Chuchu Li; Zhicang Ye

doi:10.1007/978-1-4939-3389-1_34

Immunoproteomic Approach for Screening Vaccine Candidates from Bacterial Outer Membrane Proteins

Methods Mol Biol. 2016:1404:519-528. doi: 10.1007/978-1-4939-3389-1_34.

Authors

Jianyi Pan¹, Chuchu Li², Zhicang Ye²

Affiliations

¹ Institute of Proteomics and Molecular Enzymology, School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China. jianyi.pan@zstu.edu.cn.
² Institute of Proteomics and Molecular Enzymology, School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.

PMID: 27076320
DOI: 10.1007/978-1-4939-3389-1_34

Abstract

Outer membrane proteins (OMPs) are unique to Gram-negative bacteria and have been revealed as potential vaccine candidates for conferring protection against infections in recent years. Immunoproteomics is a powerful technique that is ideally suited to screen and identify potential vaccine candidates. This chapter presents a brief outline of the screening of immunogenic OMPs from Vibrio parahaemolyticus by an immunoproteomic strategy that was based on two-dimensional electrophoresis (2-DE) and immunoblotting. The protective efficacy provided by the immunogenic OMP Vp0802 determined by active protection experiment assays is also presented in brief.

Keywords: Immunogenic; Immunoproteomics; Outer membrane protein (OMP); Protective antigen; V. parahaemolyticus; Vaccine candidate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Outer Membrane Proteins / immunology*
Bacterial Outer Membrane Proteins / metabolism*
Bacterial Vaccines / immunology*
Bacterial Vaccines / metabolism*
Immune Sera / immunology
Mice
Mice, Inbred ICR
Proteomics / methods*
Vibrio parahaemolyticus / immunology

Substances

Bacterial Outer Membrane Proteins
Bacterial Vaccines
Immune Sera