Microfluidics for effective concentration and sorting of waterborne protozoan pathogens

M Jimenez; H Bridle

doi:10.1016/j.mimet.2016.04.008

Microfluidics for effective concentration and sorting of waterborne protozoan pathogens

J Microbiol Methods. 2016 Jul:126:8-11. doi: 10.1016/j.mimet.2016.04.008. Epub 2016 Apr 10.

Authors

M Jimenez¹, H Bridle²

Affiliations

¹ Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Riccarton, Scotland, Edinburgh EH14 4AS, United Kingdom. Electronic address: Melanie.jimenez@Glasgow.ac.uk.
² Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Riccarton, Scotland, Edinburgh EH14 4AS, United Kingdom.

PMID: 27074367
DOI: 10.1016/j.mimet.2016.04.008

Abstract

We report on an inertial focussing based microfluidics technology for concentrating waterborne protozoa, achieving a 96% recovery rate of Cryptosporidium parvum and 86% for Giardia lamblia at a throughput (mL/min) capable of replacing centrifugation. The approach can easily be extended to other parasites and also bacteria.

Keywords: Concentration; Cryptosporidium; Giardia; Microfluidics; Protozoa; Recovery rate; Sample processing; Waterborne disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomedical Engineering / methods
Centrifugation
Cryptosporidium parvum / isolation & purification*
Cryptosporidium parvum / pathogenicity
Equipment Design
Giardia lamblia / isolation & purification*
Giardia lamblia / pathogenicity
Microfluidic Analytical Techniques / methods*
Microfluidics
Water / parasitology*

Substances

Water