Abstract
Cell-death can be necrosis and apoptosis. We are investigating the mechanisms regulating the cell death that occurs on treatment of mouse cancer cell-line FM3A with antitumor 5-fluoro-2'-deoxyuridine (FUdR): necrosis occurs for the original clone F28-7, and apoptosis for its variant F28-7-A. Here we report that a microRNA (miR-351) regulates the cell death pattern. The miR-351 is expressed strongly in F28-7-A but only weakly in F28-7. Induction of a higher expression of miR-351 in F28-7 by transfecting an miRNA mimic into F28-7 resulted in a change of the death mode; necrosis to apoptosis. Furthermore, transfection of an miR-351 inhibitor into F28-7-A resulted in the morphology change, apoptosis to necrosis, in this death-by-FUdR. Possible mechanism involving lamin B1 in this miR-351's regulatory action is discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents / pharmacology
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Apoptosis / drug effects*
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Apoptosis / genetics*
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Cell Death / drug effects*
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Cell Death / genetics*
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Cell Line, Tumor
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Deoxyuridine / analogs & derivatives*
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Deoxyuridine / pharmacology
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Gene Expression Profiling
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Lamin Type B / genetics
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Lamin Type B / metabolism
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Mice
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MicroRNAs / antagonists & inhibitors
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MicroRNAs / genetics*
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MicroRNAs / metabolism*
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Molecular Mimicry
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Necrosis
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Transfection
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Up-Regulation
Substances
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5-fluoro-2'-deoxyuridine
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Antineoplastic Agents
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Lamin Type B
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MIRN351 microRNA, 351
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MicroRNAs
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RNA, Messenger
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Deoxyuridine
Grants and funding
Funding provided by JSPS KAKENHI Grant Numbers 21790078 (AS (first author)), 24790216 (AS (first author)), and 26460310 (AS (first author)).