MicroRNA-351 Regulates Two-Types of Cell Death, Necrosis and Apoptosis, Induced by 5-fluoro-2'-deoxyuridine

PLoS One. 2016 Apr 12;11(4):e0153130. doi: 10.1371/journal.pone.0153130. eCollection 2016.

Abstract

Cell-death can be necrosis and apoptosis. We are investigating the mechanisms regulating the cell death that occurs on treatment of mouse cancer cell-line FM3A with antitumor 5-fluoro-2'-deoxyuridine (FUdR): necrosis occurs for the original clone F28-7, and apoptosis for its variant F28-7-A. Here we report that a microRNA (miR-351) regulates the cell death pattern. The miR-351 is expressed strongly in F28-7-A but only weakly in F28-7. Induction of a higher expression of miR-351 in F28-7 by transfecting an miRNA mimic into F28-7 resulted in a change of the death mode; necrosis to apoptosis. Furthermore, transfection of an miR-351 inhibitor into F28-7-A resulted in the morphology change, apoptosis to necrosis, in this death-by-FUdR. Possible mechanism involving lamin B1 in this miR-351's regulatory action is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology
  • Apoptosis / drug effects*
  • Apoptosis / genetics*
  • Cell Death / drug effects*
  • Cell Death / genetics*
  • Cell Line, Tumor
  • Deoxyuridine / analogs & derivatives*
  • Deoxyuridine / pharmacology
  • Gene Expression Profiling
  • Lamin Type B / genetics
  • Lamin Type B / metabolism
  • Mice
  • MicroRNAs / antagonists & inhibitors
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism*
  • Molecular Mimicry
  • Necrosis
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Transfection
  • Up-Regulation

Substances

  • 5-fluoro-2'-deoxyuridine
  • Antineoplastic Agents
  • Lamin Type B
  • MIRN351 microRNA, 351
  • MicroRNAs
  • RNA, Messenger
  • Deoxyuridine

Grants and funding

Funding provided by JSPS KAKENHI Grant Numbers 21790078 (AS (first author)), 24790216 (AS (first author)), and 26460310 (AS (first author)).