Objective: To compare the two different methods to isolate the exosome from the ascites of colorectal cancer (CRC) patient and find the efficient one.
Methods: Exosome from the ascites of CRC patient were isolated by two different methods: density gradient exosome isolation (DG-Exo) and Exo-Quick isolation, and followed by identification with transmission electron microscopy observation and Western blot analysis. And then, Nanodrop was used for protein quantification.
Results: Exosome were isolated by both of the two methods. The protein concentration of the exosome isolated by the Exo-Quick isolation were higher than that of DG-Exo.
Conclusion: Exo-Quick isolation can obtain higher purity and more complete exosome from the ascites.