Development, subcellular positioning and selective protein accumulation in the dimorphic chloroplasts of single-cell C4 species

Abstract

C4 photosynthesis is typically associated with a carbon concentrating mechanism based on close collaboration between two photosynthetic cell types (Kranz C4). Surprisingly, four species in the family Chenopodiaceae have been described, which perform all required steps for a functional and effective C4 cycle within individual photosynthetic cells. These single-cell C4 species utilize a unique subcellular compartmentation and two functionally different chloroplast types that mimic the functions of the two cell types of the Kranz C4 system. In this review, we will summarize and discuss studies on chloroplast development, positioning and selective accumulation of nuclear encoded proteins, which ultimately allow the operation of a C4 carbon concentrating mechanism within individual cells.