Genetic characterization of a densovirus isolated from great tit (Parus major) in China

Infect Genet Evol. 2016 Jul:41:107-112. doi: 10.1016/j.meegid.2016.03.035. Epub 2016 Apr 2.

Abstract

During a study of ornithophilous viruses in China, a new densovirus (DNV) was isolated from the lung tissue of Parus major (PmDNV-JL). The complete genome of PmDNV-JL was cloned and sequenced. Five open reading frames (ORFs) were identified in the 5166nt sequence, on the basis of deduced amino acids. It was further shown that this virus caused cytopathic effects (CPE) in Feline kidney cells. The NS1 gene sequence of PmDNV-JL shares 70-99% nucleotide sequence identity with isolates of the Blattella germanica densovirus (BgDNV) and BgDNV-like virus. Phylogenetic analysis indicated that the predicted amino acid sequences of capsid (VP) and non-structural domain (NS1) of PmDNV-JL clustered with the BgDNV and were similar to BgDNV-HB within the genus Densovirus.

Keywords: Densovirus; Lung tissue; Parus major; Sequence analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bird Diseases / epidemiology*
  • Bird Diseases / transmission
  • Bird Diseases / virology
  • Capsid Proteins / genetics
  • Cats
  • China / epidemiology
  • DNA, Viral / genetics
  • Densovirus / classification
  • Densovirus / genetics*
  • Densovirus / isolation & purification
  • Epithelial Cells / virology
  • Genome, Viral*
  • Kidney / virology
  • Lung / virology
  • Open Reading Frames
  • Parvoviridae Infections / epidemiology
  • Parvoviridae Infections / transmission
  • Parvoviridae Infections / veterinary*
  • Parvoviridae Infections / virology
  • Passeriformes / virology*
  • Phylogeny*
  • Sequence Analysis, DNA
  • Viral Nonstructural Proteins / genetics

Substances

  • Capsid Proteins
  • DNA, Viral
  • Viral Nonstructural Proteins