Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche

Sci Rep. 2016 Apr 4:6:23885. doi: 10.1038/srep23885.

Abstract

While implicated in therapeutic resistance, malignant progenitor cell cycle kinetics have been difficult to quantify in real-time. We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle indicator reporter (Fucci2BL) to image live single progenitors on a defined niche coupled with cell cycle gene expression analysis. We have identified key differences in cell cycle regulatory gene expression and transit times between normal and chronic myeloid leukemia progenitors that may inform cancer stem cell eradication strategies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle
  • Cell Line
  • Coculture Techniques
  • Drug Resistance, Neoplasm
  • Gene Expression Profiling / methods*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Kinetics
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / diagnostic imaging*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism
  • Microscopy, Confocal
  • Neoplastic Stem Cells / metabolism*
  • Sequence Analysis, RNA / methods*
  • Stem Cell Niche*
  • Time-Lapse Imaging