Methods for Assessing the In Vivo Role of PTEN in Glucose Homeostasis

Cynthia T Luk; Stephanie A Schroer; Minna Woo

doi:10.1007/978-1-4939-3299-3_7

Methods for Assessing the In Vivo Role of PTEN in Glucose Homeostasis

Methods Mol Biol. 2016:1388:75-91. doi: 10.1007/978-1-4939-3299-3_7.

Authors

Cynthia T Luk^{1

2}, Stephanie A Schroer¹, Minna Woo^{3

4

5

6}

Affiliations

¹ Toronto General Research Institute, University Health Network, Toronto, ON, Canada, M5G 2C4.
² Institute of Medical Science, University of Toronto, Toronto, ON, Canada, M5G 2C4.
³ Toronto General Research Institute, University Health Network, Toronto, ON, Canada, M5G 2C4. mwoo@uhnresearch.ca.
⁴ Institute of Medical Science, University of Toronto, Toronto, ON, Canada, M5G 2C4. mwoo@uhnresearch.ca.
⁵ Division of Endocrinology, Department of Medicine, University Health Network, University of Toronto, Toronto, ON, Canada, M5G 2C4. mwoo@uhnresearch.ca.
⁶ Toronto General Research Institute, 101 College Street, MaRS Centre/TMDT, Room 10-363, Toronto, ON, Canada, M5G 1L7. mwoo@uhnresearch.ca.

PMID: 27033072
DOI: 10.1007/978-1-4939-3299-3_7

Abstract

PTEN plays an important role in diabetes pathogenesis not only as a key negative regulator of the PI3K/Akt pathway required for insulin action, but also via its role in other cell processes required to maintain metabolic homeostasis. We describe the generation of tissue-specific PTEN knockout mice and models of both type 1 and type 2 diabetes, which we have found useful for the study of diabetes pathogenesis. We also outline common methods suitable for the characterization of glucose homeostasis in rodent models, including techniques to measure beta cell function and insulin sensitivity.

Keywords: Diabetes; Glucose; Insulin sensitivity; PTEN.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Crystallography, X-Ray
Diabetes Mellitus / metabolism*
Disease Models, Animal
Glucose / metabolism*
Homeostasis
Humans
Insulin / metabolism
Mice
PTEN Phosphohydrolase / metabolism*

Substances

Insulin
PTEN Phosphohydrolase
Glucose

Grants and funding

Canadian Institutes of Health Research/Canada