Abstract
A molecular biosensor based on DNA aptamers (aptasensor) for the diagnosis of lung cancer in blood plasma samples was designed. To create the aptasensor, the aptamer 17_80, obtained in the study of postoperative material, was used. The affinity and binding selectivity of the aptamer 17_80 to the lung tumor tissue was confirmed on histological sections of postmortem samples of lung tissue. Using affinity enrichment and mass spectrometry, a possible target molecule of the aptamer 17_80, vimentin, was found.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Aptamers, Nucleotide / chemistry*
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Aptamers, Nucleotide / pharmacology
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Biomarkers, Tumor / blood*
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Biomarkers, Tumor / metabolism
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Biosensing Techniques / methods*
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Carcinoma, Squamous Cell / blood*
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Early Detection of Cancer / methods*
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Humans
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Lung Neoplasms / blood*
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Middle Aged
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Neoplasm Proteins / blood*
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Neoplasm Proteins / metabolism
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Oncogene Proteins
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Protein Binding
Substances
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Aptamers, Nucleotide
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Biomarkers, Tumor
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Neoplasm Proteins
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Oncogene Proteins