In this work, an endosulfan-degrading strain was isolated from the aged soil contaminated by endosulfan, and identified as Ochrobactrum sp. EB-4 by 16S rDNA sequence analysis. The microbial degradation characteristics of endosulfan in three eluents (Tween 80 + SDS, Tween 80 + Na2SiO3, Tween 80 + SDS + Na2SiO3) were investigated. The results showed that the degradation percents of α-, β-endosulfan in the three eluents were 86.83 %∼92.91 % and 88.90 %∼93.94 % in 15 days, respectively. The degradation process can be well described by the first-order kinetic model, and the half-times of α-endosulfan in eluent 1∼eluent 3 were 3.83, 5.29, and 4.53 days, while those of β-endosulfan were 3.35, 4.50 and 3.79 days, respectively. The endosulfan diol and endosulfan sulfate as main metabolites were detected, and the former can be further degraded by this strain, which revealed that the simultaneously happened hydrolysis and oxidation reactions were the main degradation processes, and dominated by hydrolysis reaction. After 5 days of washing with the eluents, 56.00∼84.33 % of α-endosulfan, and 46.49∼68.56 % of β-endosulfan in soil were eluted, respectively, and can be entirely biodegraded in 12 days, which indicated that the microbial degradation was the rate-determining step.
Keywords: Elution; Endosulfan; Microbial degradation; Soil; Surfactant.