Effects of plasma irradiation using various feeding gases on growth of Raphanus sativus L

Thapanut Sarinont; Takaaki Amano; Pankaj Attri; Kazunori Koga; Nobuya Hayashi; Masaharu Shiratani

doi:10.1016/j.abb.2016.03.024

Effects of plasma irradiation using various feeding gases on growth of Raphanus sativus L

Arch Biochem Biophys. 2016 Sep 1:605:129-40. doi: 10.1016/j.abb.2016.03.024. Epub 2016 Mar 26.

Authors

Thapanut Sarinont¹, Takaaki Amano¹, Pankaj Attri², Kazunori Koga¹, Nobuya Hayashi³, Masaharu Shiratani⁴

Affiliations

¹ Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
² Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul 139-701, South Korea.
³ Interdisciplinary Graduate School of Engineering Science, Kyushu University, 6-1 Kasuga-kouen, Fukuoka 816-8580, Japan.
⁴ Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: siratani@ed.kyushu-u.ac.jp.

PMID: 27021583
DOI: 10.1016/j.abb.2016.03.024

Abstract

In this work, we have studied the action of dielectric barrier discharge (DBD) plasma irradiation using various feeding gases on seeds of Raphanus sativus L. and analysis their growth. Our experimental data shows that Air, O2, and NO(10%)+N2 feeding gases plasma irradiation enhanced plant growth, whereas N2, He and Ar feeding gases plasma irradiation had little influence on plant growth. Moreover, humid air plasma irradiation was more effective in growth enhancement than dry one. More than 2.3 times faster growth was observed by 3 min air plasma irradiation with 40-90% relative humidity. The reactive species generated by plasma in gas phase were detected using optical emission spectroscopy and in liquid phase by electron spin resonance (ESR) spectroscopy. We concluded that OH and O radicals were key species for plant growth enhancement.

Keywords: Gas fumigation; Humidity; Plant growth enhancement; Plasma; Radish sprout; Raphanus sativus L.; Seed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Air
Electromagnetic Fields
Electron Spin Resonance Spectroscopy
Humidity
Hydroxyl Radical / chemistry
Nitrogen / chemistry
Oxygen / chemistry
Plasma Gases*
Raphanus / growth & development*
Reactive Oxygen Species / chemistry
Seeds / growth & development*

Substances

Plasma Gases
Reactive Oxygen Species
Hydroxyl Radical
Nitrogen
Oxygen