Detection and Characteristics of Rifampicin-Resistant Isolates of Mycobacterium tuberculosis

A G Cherednichenko; M A Dymova; O A Solodilova; T I Petrenko; A I Prozorov; M L Filipenko

doi:10.1007/s10517-016-3243-3

Detection and Characteristics of Rifampicin-Resistant Isolates of Mycobacterium tuberculosis

Bull Exp Biol Med. 2016 Mar;160(5):659-63. doi: 10.1007/s10517-016-3243-3. Epub 2016 Mar 29.

Authors

A G Cherednichenko¹, M A Dymova^{2

3}, O A Solodilova⁴, T I Petrenko¹, A I Prozorov⁴, M L Filipenko^{5

6}

Affiliations

¹ Research Institute of Tuberculosis, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
² Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. maya.a.rot@gmail.com.
³ Novosibirsk State University, Novosibirsk, Russia. maya.a.rot@gmail.com.
⁴ Altai Regional Antituberculous Dispensary, Barnaul, Russia.
⁵ Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
⁶ Novosibirsk State University, Novosibirsk, Russia.

PMID: 27021095
DOI: 10.1007/s10517-016-3243-3

Abstract

Genotyping and analysis the drug resistance of 59 isolates of M. tuberculosis obtained from patients living in Altai Territory were performed using a BACTEC MGIT 960 fluorometric system by means of VNTR typing (variable number tandem repeat), PCR-RFLP analysis, and sequence analysis. The occurrence frequency was highest for isolates of the Beijing family (n=30, 50.8%). Analysis of mutation spectrum in the rpoB gene associated with rifampicin resistance revealed the major mutation (codon 531 of the rpoB gene) in 93% samples, which allows us to use rapid test systems.

Keywords: Mycobacterium tuberculosis; drug resistance; rifampicin; rpoB gene.

MeSH terms

Adult
Antitubercular Agents / pharmacology*
Bacterial Proteins / genetics*
DNA-Directed RNA Polymerases / genetics*
Drug Resistance, Multiple, Bacterial / genetics*
Humans
Male
Microbial Sensitivity Tests
Middle Aged
Minisatellite Repeats / genetics
Molecular Typing
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / genetics*
Mycobacterium tuberculosis / isolation & purification
Polymorphism, Restriction Fragment Length
Rifampin / pharmacology*
Tuberculosis, Pulmonary / diagnosis*
Tuberculosis, Pulmonary / drug therapy
Tuberculosis, Pulmonary / microbiology
Young Adult

Substances

Antitubercular Agents
Bacterial Proteins
rpoB protein, Mycobacterium tuberculosis
DNA-Directed RNA Polymerases
Rifampin