Glycan multivalency effects toward albumin enable N-glycan-dependent tumor targeting

Akihiro Ogura; Tsuyoshi Tahara; Satoshi Nozaki; Hirotaka Onoe; Almira Kurbangalieva; Yasuyoshi Watanabe; Katsunori Tanaka

doi:10.1016/j.bmcl.2016.03.046

Glycan multivalency effects toward albumin enable N-glycan-dependent tumor targeting

Bioorg Med Chem Lett. 2016 May 1;26(9):2251-4. doi: 10.1016/j.bmcl.2016.03.046. Epub 2016 Mar 14.

Authors

Akihiro Ogura¹, Tsuyoshi Tahara², Satoshi Nozaki², Hirotaka Onoe², Almira Kurbangalieva³, Yasuyoshi Watanabe², Katsunori Tanaka⁴

Affiliations

¹ Biofunctional Synthetic Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
² RIKEN Center for Life Science Technologies, 6-7-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan.
³ Biofunctional Chemistry Laboratory, A. Butlerov Institute of Chemistry, Kazan Federal University, 18 Kremlyovskaya Street, Kazan 420008, Russia.
⁴ Biofunctional Synthetic Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan; Biofunctional Chemistry Laboratory, A. Butlerov Institute of Chemistry, Kazan Federal University, 18 Kremlyovskaya Street, Kazan 420008, Russia; Japan Science and Technology Agency-PRESTO, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. Electronic address: kotzenori@riken.jp.

PMID: 27020300
DOI: 10.1016/j.bmcl.2016.03.046

Abstract

Multivalent interactions play an essential role in molecular recognition in living systems. These effects were employed to target tumor cells using albumin clusters bearing ∼10 molecules of asparagine-linked glycans (N-glycans). Noninvasive near-infrared fluorescence imaging clearly revealed A431 tumors implanted in BALB/cA-nu/nu mice after 1h in an N-glycan structure-dependent manner, thereby demonstrating the efficient use of glycan multivalency effects for tumor targeting in vivo.

Keywords: A431 tumor; In vivo molecular imaging; Multivalency effects; N-Glycan; α(2,3)-Disialoglycan.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Albumins / metabolism*
Animals
Carbohydrate Sequence
Cell Line, Tumor
Heterografts
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasms / metabolism*
Neoplasms / pathology
Polysaccharides / chemistry
Polysaccharides / metabolism*

Substances

Albumins
Polysaccharides