Effects of chlorpyrifos on the gut microbiome and urine metabolome in mouse (Mus musculus)

Yanping Zhao; Yan Zhang; Guoxiang Wang; Ruiming Han; Xianchuan Xie

doi:10.1016/j.chemosphere.2016.03.055

Effects of chlorpyrifos on the gut microbiome and urine metabolome in mouse (Mus musculus)

Chemosphere. 2016 Jun:153:287-93. doi: 10.1016/j.chemosphere.2016.03.055. Epub 2016 Mar 26.

Authors

Yanping Zhao¹, Yan Zhang², Guoxiang Wang³, Ruiming Han¹, Xianchuan Xie⁴

Affiliations

¹ Jiangsu Key Laboratory of Environmental Change and Ecological Construction, Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, School of Geographical Science, Nanjing Normal University, Nanjing 210023, China.
² State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.
³ Jiangsu Key Laboratory of Environmental Change and Ecological Construction, Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, School of Geographical Science, Nanjing Normal University, Nanjing 210023, China. Electronic address: wangguoxiang@njnu.edu.cn.
⁴ State Key Laboratory of Pollution Control and Resource Reuse, Center for Hydrosciences Research, School of the Environment, Nanjing University, Nanjing 210023, China.

PMID: 27018521
DOI: 10.1016/j.chemosphere.2016.03.055

Abstract

In this study, the toxic effects of clorpyrifos (CPF) on the gut microbiome and related urine metabolome in mouse (Mus musculus) were investigated. Mice were exposed to a daily dose of 1 mg kg(-1) bodyweight of CPF for 30 d. As a result, CPF significantly altered the gut microbiota composition in terms of the relative abundance of key microbes. Meanwhile, CPF exposure induced the alterations of urine metabolites related to the metabolism of amino acids, energy, short-chain fatty acids (SCFAs), phenyl derivatives and bile acids. High correlations were observed between perturbed gut microbiome and altered metabolic profiles. These perturbations finally resulted in intestinal inflammation and abnormal intestinal permeability, which were also confirm by the histologic changes in colon and remarkable increase of lipopolysaccharide (LPS) and diamine oxidase (DAO) in the serum of CPF-treated mice. Our findings will provide a new perspective to reveal the mechanism of CPF toxicity.

Keywords: Chlorpyrifos; Gut microbiome; High-throughput sequencing; Metabolomics; Mice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amine Oxidase (Copper-Containing) / blood
Amino Acids / metabolism
Animals
Bile Acids and Salts / metabolism
Chlorpyrifos / toxicity*
Fatty Acids, Volatile / metabolism
Gastrointestinal Microbiome / drug effects*
Intestines / drug effects*
Intestines / microbiology
Lipopolysaccharides / blood
Male
Metabolome / drug effects
Mice
Urine / chemistry*

Substances

Amino Acids
Bile Acids and Salts
Fatty Acids, Volatile
Lipopolysaccharides
Amine Oxidase (Copper-Containing)
Chlorpyrifos