p-Coumaric acid, a novel and effective biomarker for quantifying hypoxic stress by HILIC-ESI-MS

Yuliya E Silina; Claudia Fink-Straube; Rainer G Hanselmann; Henrike Peuschel; Dietrich A Volmer

doi:10.1016/j.jchromb.2016.03.005

p-Coumaric acid, a novel and effective biomarker for quantifying hypoxic stress by HILIC-ESI-MS

J Chromatogr B Analyt Technol Biomed Life Sci. 2016 May 1:1020:6-13. doi: 10.1016/j.jchromb.2016.03.005. Epub 2016 Mar 9.

Authors

Yuliya E Silina¹, Claudia Fink-Straube², Rainer G Hanselmann², Henrike Peuschel², Dietrich A Volmer³

Affiliations

¹ INM-Leibniz Institute for New Materials, Campus D2 2, 66123 Saarbrücken, Germany; Institute of Bioanalytical Chemistry, Saarland University, Campus B2.2, 66123 Saarbrücken, Germany. Electronic address: yuliya.silina@leibniz-inm.de.
² INM-Leibniz Institute for New Materials, Campus D2 2, 66123 Saarbrücken, Germany.
³ Institute of Bioanalytical Chemistry, Saarland University, Campus B2.2, 66123 Saarbrücken, Germany.

PMID: 27010352
DOI: 10.1016/j.jchromb.2016.03.005

Abstract

In this study, we report p-coumaric acid as novel and effective response marker for indirectly measuring the levels of hypoxia in normal primary bronchial epithelial cells. We developed a simple and rapid technique based on hydrophilic interaction chromatography-electrospray ionization-mass spectrometry (HILIC-ESI-MS). During 168h of hypoxia without induction of reactive oxygen species (ROS), an almost linear increase of p-coumaric acid levels was observed. We interpret the increasing p-coumaric acid concentrations during hypoxia as a result of cell damage, triggered by reduced co-enzyme Q10 levels, because the oxidative cascade was not able to supply sufficient energy. The HILIC-ESI-MS assay within p-coumaric acid exhibited a linear dynamic range from 60 to 610 ng/μL with correlation coefficient of 0.9998. The precision of the assay was ≤15% RSD and method accuracies between 97 and 108%.

Keywords: Cellular hypoxia; Co-enzyme Q10; HILIC-ESI–MS; ROS; p-Coumaric acid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins / analysis
Actins / metabolism
Biomarkers / analysis*
Biomarkers / metabolism
Cell Hypoxia / physiology*
Cell Nucleus / chemistry
Cell Nucleus / metabolism
Cells, Cultured
Chromatography, High Pressure Liquid / methods*
Coumaric Acids / analysis*
Coumaric Acids / metabolism
Humans
Hydrophobic and Hydrophilic Interactions
Hypoxia-Inducible Factor 1, alpha Subunit / analysis
Hypoxia-Inducible Factor 1, alpha Subunit / metabolism
Oxidative Stress / physiology*
Propionates
Reproducibility of Results
Respiratory Mucosa / cytology
Spectrometry, Mass, Electrospray Ionization / methods*
Ubiquinone / analogs & derivatives

Substances

Actins
Biomarkers
Coumaric Acids
HIF1A protein, human
Hypoxia-Inducible Factor 1, alpha Subunit
Propionates
Ubiquinone
coenzyme Q10
p-coumaric acid