Conjugated linoleic acids suppress inflammatory response and ICAM-1 expression through inhibition of NF-κB and MAPK signaling in human bronchial epithelial cells

Wen-Chung Huang; Rong-Syuan Tu; Ya-Ling Chen; Yun-Yun Tsai; Chwan-Fwu Lin; Chian-Jiun Liou

doi:10.1039/c5fo01037c

Conjugated linoleic acids suppress inflammatory response and ICAM-1 expression through inhibition of NF-κB and MAPK signaling in human bronchial epithelial cells

Food Funct. 2016 Apr;7(4):2025-33. doi: 10.1039/c5fo01037c.

Authors

Wen-Chung Huang¹, Rong-Syuan Tu², Ya-Ling Chen², Yun-Yun Tsai³, Chwan-Fwu Lin⁴, Chian-Jiun Liou⁵

Affiliations

¹ Graduate Institute of Health Industry Technology and Research Center for Industry of Human Ecology, College of Human Ecology, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan and Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Guishan Dist., Taoyuan City 33303, Taiwan. ccliu@gw.cgust.edu.tw.
² Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan.
³ Graduate Institute of Health Industry Technology and Research Center for Industry of Human Ecology, College of Human Ecology, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan and Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, No. 70, Dazhi St., Zhongshan Dist., Taipei City 104, Taiwan.
⁴ Department of Cosmetic Science, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan.
⁵ Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Guishan Dist., Taoyuan City 33303, Taiwan. ccliu@gw.cgust.edu.tw and Department of Nursing, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan.

PMID: 27007063
DOI: 10.1039/c5fo01037c

Abstract

Conjugated linoleic acids (CLAs) comprise a group of natural unsaturated fatty acids. CLA was reported to have anti-asthma, anti-adiposity, and anti-tumor effects. The present study aimed to evaluate the suppressive effects of cis-9, trans-11-CLA (c9,t11-CLA) on the expression of proinflammatory cytokines and intercellular adhesion molecule 1 (ICAM-1) in TNF-α-stimulated human bronchial epithelial (BEAS-2B) cells. After treating with various doses of c9,t11-CLA (12.5-100 μg ml(-1)), BEAS-2B cells were induced into an inflamed state by adding TNF-α or TNF-α/IL-4. The presence of c9,t11-CLA significantly suppressed the secretion of cytokines IL-6, IL-8, CCL5, and MCP-1. We also found that c9,t11-CLA inhibited ICAM-1 expression, and decreased monocyte adhesion to inflamed bronchial epithelial cells. Interestingly, c9,t11-CLA attenuated the phosphorylation of mitogen-activated protein kinase (MAPK) and down-regulated the activation of nuclear factor-κB (NF-κB). These results suggested that the anti-inflammatory effects of c9,t11-CLA were mediated by inhibiting proinflammatory cytokines, chemokines, and ICAM-1 expression by blocking NF-κB transcription regulation and by attenuating MAPK signaling pathways.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bronchi / cytology
Bronchi / drug effects*
Bronchi / immunology
Cell Line
Epithelial Cells / drug effects
Epithelial Cells / immunology
Humans
Intercellular Adhesion Molecule-1 / genetics*
Intercellular Adhesion Molecule-1 / immunology
Interleukin-6 / genetics
Interleukin-6 / immunology
Interleukin-8 / genetics
Interleukin-8 / immunology
Linoleic Acids, Conjugated / pharmacology*
Mitogen-Activated Protein Kinases / genetics*
Mitogen-Activated Protein Kinases / immunology
Monocytes / drug effects
Monocytes / immunology
NF-kappa B / genetics*
NF-kappa B / immunology
Signal Transduction / drug effects
Tumor Necrosis Factor-alpha / genetics
Tumor Necrosis Factor-alpha / immunology

Substances

Interleukin-6
Interleukin-8
Linoleic Acids, Conjugated
NF-kappa B
Tumor Necrosis Factor-alpha
Intercellular Adhesion Molecule-1
Mitogen-Activated Protein Kinases