Bioindustrial wastewaters, often characterised by high carbon and nitrogen contents, have shown promise as a valuable resource for the cultivation of beneficial microorganisms. The purpose of this study was to assess if Parachlorella kessleri could utilise brewery wastewater (Br WW) for growth and production of metabolites. P. kessleri was cultivated on different concentrations of Br WW over 14 days. Higher concentrations of Br WW led to an approximate two-fold increase in dry cell weight yielding a maximum of 12.3 g DCW/L. High glucose and nitrogen utilisation was associated with high algal biomass yields, with a 97% reduction in glucose achieved in 50% (v/v) Br WW cultures after 14 days. Assessing the benefits to P. kessleri, increases in oleic and α-linoleic acids were seen in 50 and 10% (v/v) Br WW cultures. Concentration of Br WW did not have an impact on the overall antioxidant activities of microalgal cultures, however, it did affect phenolic levels (2.4-fold increase) in 50% (v/v) Br WW cultures. This research demonstrated that P. kessleri did utilise the carbon and nitrogen content in the Br WW for growth and metabolite production, thereby reducing the nutrient load of the Br WW.