Preparation of photonic-magnetic responsive molecularly imprinted microspheres and their application to fast and selective extraction of 17β-estradiol

Hailong Peng; Mei Luo; Hua Xiong; Ningxiang Yu; Fangjian Ning; Jieping Fan; Zheling Zeng; Jinhua Li; Lingxin Chen

doi:10.1016/j.chroma.2016.03.003

Preparation of photonic-magnetic responsive molecularly imprinted microspheres and their application to fast and selective extraction of 17β-estradiol

J Chromatogr A. 2016 Apr 15:1442:1-11. doi: 10.1016/j.chroma.2016.03.003. Epub 2016 Mar 4.

Authors

Hailong Peng¹, Mei Luo¹, Hua Xiong², Ningxiang Yu³, Fangjian Ning³, Jieping Fan⁴, Zheling Zeng⁴, Jinhua Li⁵, Lingxin Chen⁶

Affiliations

¹ School of Resources, Environmental, and Chemical Engineering, Nanchang University, Nanchang 330031, China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
² State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: huaxiong100@126.com.
³ State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
⁴ School of Resources, Environmental, and Chemical Engineering, Nanchang University, Nanchang 330031, China.
⁵ Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
⁶ Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China. Electronic address: lxchen@yic.ac.cn.

PMID: 27000739
DOI: 10.1016/j.chroma.2016.03.003

Abstract

Photonic-magnetic responsive molecularly imprinted microspheres (PM-MIMs) were prepared by seed polymerization, through suitable functionalization of magnetic nanoparticles for further coating with photoresponsive functional monomer and imprinted layers, and then were successfully applied to the fast and selective extraction of 17β-estradiol (17β-E2) from real samples. The PM-MIMs possessed a sandwich micro-spherical structure containing Fe3O4 core, SiO2 middle layer, and MIPs shell with thickness of 25 nm. The PM-MIMs displayed excellent photoresponsive properties and could be rapidly separated from solutions under an external magnet. The PM-MIMs had specific affinity towards 17β-E2 with high adsorption capacity (Qmax=0.84 mg g(-1)) and fast binding kinetics (Kd=26.08 mg L(-1)). The PM-MIMs proved to be an ideal photoswitch with the ability of reversible uptake and release of 17β-E2 upon alternate 365 and 440 nm irradiation: 45.0% of 17β-E2 released from the PM-MIMs upon 365 nm irradiation, and 94.0% of the released 17β-E2 was rebound to the PM-MIMs at 440 nm. Accordingly, the PM-MIMs were applied for fast separation and extraction of 17β-E2 followed by HPLC-UV determination, presenting the low limit of detection (LOD, S/N=3) and quantification (LOQ, S/N=10) of 0.18 and 0.62 μmol L(-1), respectively. The high recoveries for spiked milk powder and drinking water samples were in the range of 97.5-113.0% with relative standard deviations less than 4.4%. This study reasonably combined photonic response, magnetic separation and surface imprinting, which endowed the PM-MIMs with significant advantages of high adsorption capacity and fast binding kinetics, convenient separation and recycled use, and simple rapid eco-benign adsorption/elution processes for template molecules. Thus, the PM-MIMs based method may be a simple, rapid, convenient, cost-effective and environmentally-friendly way for simultaneous separation, enrichment and detection of trace 17β-E2 in complicated matrices.

Keywords: 17β-Estradiol; Magnetic separation; Molecularly imprinted polymers; Photonic responsive.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Chemistry Techniques, Analytical / methods*
Estradiol / chemistry
Estradiol / isolation & purification*
Magnetics*
Microspheres*
Molecular Imprinting
Nanoparticles / chemistry
Polymerization
Silicon Dioxide / chemistry

Substances

Estradiol
Silicon Dioxide