Abstract
To quantitatively evaluate the therapeutic effects of diosgenin (DG) and investigate the role of IL-4 on skin inflammation, alterations in luciferase-derived signal and general phenotype biomarkers were measured in IL-4/Luc/CNS-1 transgenic mice with phthalic anhydride (PA)-induced skin inflammation after treatment with DG for 4 weeks. High levels of luciferase-derived signal detected in the abdominal region and submandibular lymph node (SL) of the PA treated group was significantly decreased by 67-88% in the PA + DG cotreated group. Furthermore, the weight of the lymph node and spleen, IgE concentration, epidermis thickness, and number of infiltrated mast cells were lower in the PA + DG treated group than the PA + Vehicle treated group. Moreover, expression of IL-6 and vascular endothelial growth factor (VEGF) also decreased in the PA + DG cotreated group. These results suggest that PA-induced skin inflammation could be successfully suppressed by DG treatment in IL-4/Luc/CNS-1 Tg mice through attenuation of IL-4 and IL-6 expression, as well as decreased IgE concentration and mast cells infiltration.
Keywords:
IL-4/Luc/CNS-1 transgenic mice; IgE; diosgenin; phthalic anhydride; skin inflammation.
MeSH terms
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Animals
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Anti-Inflammatory Agents / pharmacology*
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Cell Movement / drug effects
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Dermatitis, Atopic / chemically induced
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Dermatitis, Atopic / drug therapy*
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Dermatitis, Atopic / immunology
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Dermatitis, Atopic / pathology
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Diosgenin / pharmacology*
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Gene Expression
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Genes, Reporter
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Immunoglobulin E / genetics
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Interleukin-4 / antagonists & inhibitors
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Interleukin-4 / genetics*
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Interleukin-4 / immunology
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Interleukin-6 / antagonists & inhibitors
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Interleukin-6 / genetics*
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Interleukin-6 / immunology
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Luciferases / genetics
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Luciferases / immunology
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Lymph Nodes / drug effects
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Lymph Nodes / immunology
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Lymph Nodes / pathology
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Mast Cells / drug effects
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Mast Cells / immunology
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Mast Cells / pathology
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Mice
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Mice, Transgenic
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Organ Size / drug effects
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Phthalic Anhydrides
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Skin / drug effects*
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Skin / immunology
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Skin / pathology
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Spleen / drug effects
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Spleen / immunology
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Spleen / pathology
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Vascular Endothelial Growth Factor A / genetics
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Vascular Endothelial Growth Factor A / immunology
Substances
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Anti-Inflammatory Agents
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Interleukin-6
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Phthalic Anhydrides
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Vascular Endothelial Growth Factor A
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interleukin-6, mouse
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vascular endothelial growth factor A, mouse
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Interleukin-4
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Immunoglobulin E
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Luciferases
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Diosgenin
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phthalic anhydride