Discriminative power of Campylobacter phenotypic and genotypic typing methods

Alexandra Duarte; Tomasz Seliwiorstow; William G Miller; Lieven De Zutter; Mieke Uyttendaele; Katelijne Dierick; Nadine Botteldoorn

doi:10.1016/j.mimet.2016.03.004

Discriminative power of Campylobacter phenotypic and genotypic typing methods

J Microbiol Methods. 2016 Jun:125:33-9. doi: 10.1016/j.mimet.2016.03.004. Epub 2016 Mar 18.

Authors

Alexandra Duarte¹, Tomasz Seliwiorstow², William G Miller³, Lieven De Zutter⁴, Mieke Uyttendaele⁵, Katelijne Dierick⁶, Nadine Botteldoorn⁶

Affiliations

¹ Laboratory of Food Microbiology and Food Preservation, Dept. Food Safety & Food Quality Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium; Institute of Public Health, Scientific service Food-borne pathogens, Juliette Wytsman Street 14, 1050 Brussels, Belgium.
² Laboratory of Food Microbiology and Food Preservation, Dept. Food Safety & Food Quality Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium; Dept. Veterinary Public Health & Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
³ USDA, ARS, WRRC, Produce Safety and Microbiology, 800 Buchanan Street, Alabany, CA, 94710, USA.
⁴ Dept. Veterinary Public Health & Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
⁵ Laboratory of Food Microbiology and Food Preservation, Dept. Food Safety & Food Quality Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium.
⁶ Institute of Public Health, Scientific service Food-borne pathogens, Juliette Wytsman Street 14, 1050 Brussels, Belgium.

PMID: 26996762
DOI: 10.1016/j.mimet.2016.03.004

Abstract

The aim of this study was to compare different typing methods, individually and combined, for use in the monitoring of Campylobacter in food. Campylobacter jejuni (n=94) and Campylobacter coli (n=52) isolated from different broiler meat carcasses were characterized using multilocus sequence typing (MLST), flagellin gene A restriction fragment length polymorphism typing (flaA-RFLP), antimicrobial resistance profiling (AMRp), the presence/absence of 5 putative virulence genes; and, exclusively for C. jejuni, the determination of lipooligosaccharide (LOS) class. Discriminatory power was calculated by the Simpson's index of diversity (SID) and the congruence was measured by the adjusted Rand index and adjusted Wallace coefficient. MLST was individually the most discriminative typing method for both C. jejuni (SID=0.981) and C. coli (SID=0.957). The most discriminative combination with a SID of 0.992 for both C. jejuni and C. coli was obtained by combining MLST with flaA-RFLP. The combination of MLST with flaA-RFLP is an easy and feasible typing method for short-term monitoring of Campylobacter in broiler meat carcass.

Keywords: Antimicrobial resistance; Broiler meat; Campylobacter coli; Campylobacter jejuni; Food; MLST; Typing methods; flaA-RFLP.

Publication types

Comparative Study

MeSH terms

Animals
Bacterial Typing Techniques / methods*
Bacterial Typing Techniques / standards
Campylobacter coli / classification*
Campylobacter coli / genetics*
Campylobacter coli / isolation & purification
Campylobacter jejuni / classification*
Campylobacter jejuni / genetics*
Campylobacter jejuni / isolation & purification
Drug Resistance, Multiple, Bacterial / genetics
Flagellin / genetics
Food Microbiology / methods*
Genotyping Techniques / methods
Genotyping Techniques / standards
Lipopolysaccharides / classification
Meat / microbiology*
Multilocus Sequence Typing / methods
Phenotype
Polymorphism, Restriction Fragment Length
Poultry / microbiology
Virulence Factors / genetics

Substances

Lipopolysaccharides
Virulence Factors
lipid-linked oligosaccharides
Flagellin