Exploring bitterness of traditional Chinese medicine samples by potentiometric electronic tongue and by capillary electrophoresis and liquid chromatography coupled to UV detection

Irina Yaroshenko; Dmitry Kirsanov; Lyudmila Kartsova; Alla Sidorova; Qiyong Sun; Haitong Wan; Yu He; Ping Wang; Andrey Legin

doi:10.1016/j.talanta.2016.01.058

Exploring bitterness of traditional Chinese medicine samples by potentiometric electronic tongue and by capillary electrophoresis and liquid chromatography coupled to UV detection

Talanta. 2016 May 15:152:105-11. doi: 10.1016/j.talanta.2016.01.058. Epub 2016 Jan 28.

Authors

Irina Yaroshenko¹, Dmitry Kirsanov², Lyudmila Kartsova³, Alla Sidorova⁴, Qiyong Sun⁵, Haitong Wan⁶, Yu He⁶, Ping Wang⁵, Andrey Legin⁷

Affiliations

¹ Institute of Chemistry, St. Petersburg State University, Universitetskaya nab. 7/9, Mendeleev Center, 199034 St. Petersburg, Russia; Bioanalytical Laboratory CSU"Analytical Spectrometry", St. Petersburg State Polytechnical University, Box 27, Gzhatskaya str. 27, 198220 St. Petersburg, Russia; Laboratory of Artificial Sensory Systems, ITMO University, Kronverkskiy pr., 49, 197101 St. Petersburg, Russia.
² Institute of Chemistry, St. Petersburg State University, Universitetskaya nab. 7/9, Mendeleev Center, 199034 St. Petersburg, Russia; Laboratory of Artificial Sensory Systems, ITMO University, Kronverkskiy pr., 49, 197101 St. Petersburg, Russia. Electronic address: d.kirsanov@gmail.com.
³ Institute of Chemistry, St. Petersburg State University, Universitetskaya nab. 7/9, Mendeleev Center, 199034 St. Petersburg, Russia.
⁴ Bioanalytical Laboratory CSU"Analytical Spectrometry", St. Petersburg State Polytechnical University, Box 27, Gzhatskaya str. 27, 198220 St. Petersburg, Russia.
⁵ Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang Province 310027, China.
⁶ Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang, Hangzhou, Zhejiang Province 310053, China.
⁷ Institute of Chemistry, St. Petersburg State University, Universitetskaya nab. 7/9, Mendeleev Center, 199034 St. Petersburg, Russia; Laboratory of Artificial Sensory Systems, ITMO University, Kronverkskiy pr., 49, 197101 St. Petersburg, Russia.

PMID: 26992500
DOI: 10.1016/j.talanta.2016.01.058

Abstract

Instrumental bitterness assessment of traditional Chinese herbal medicine (TCM) preparations was addressed in this study. Three different approaches were evaluated, high-performance liquid chromatography coupled to UV detector (HPLC), capillary electrophoresis coupled to UV detector (CE) and a potentiometric multisensor system - electronic tongue (ET). Most studies involving HPLC and CE separations use these as selective instruments for quantification of individual substances. However we employed these techniques to provide chromatographic or electrophoretic sample profiles. These profiles are somewhat analogous to the profiles produced by the ET. Profiles from all instruments were then related to professional sensory panel evaluations using projections on latent structures (PLS) regression. It was found that all three methods allow for bitterness assessment in TCM samples in terms of human sensory panel with root mean squared errors of prediction ca. 0.9 within bitterness scale from 0 (no bitterness) to 6 (maximal bitterness).

Keywords: Bitterness; Chromatographic and electrophoretic profiles; Electronic tongue; Polyphenols; Traditional Chinese Medicine (TCM).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomimetics / instrumentation*
Calibration
Chromatography, High Pressure Liquid
Electrical Equipment and Supplies*
Electrophoresis, Capillary*
Medicine, Chinese Traditional*
Potentiometry / instrumentation*
Spectrophotometry, Ultraviolet
Taste*
Tongue*