H2 -Fueled ATP Synthesis on an Electrode: Mimicking Cellular Respiration

Angew Chem Int Ed Engl. 2016 May 17;55(21):6216-20. doi: 10.1002/anie.201600752. Epub 2016 Mar 15.

Abstract

ATP, the molecule used by living organisms to supply energy to many different metabolic processes, is synthesized mostly by the ATPase synthase using a proton or sodium gradient generated across a lipid membrane. We present evidence that a modified electrode surface integrating a NiFeSe hydrogenase and a F1 F0 -ATPase in a lipid membrane can couple the electrochemical oxidation of H2 to the synthesis of ATP. This electrode-assisted conversion of H2 gas into ATP could serve to generate this biochemical fuel locally when required in biomedical devices or enzymatic synthesis of valuable products.

Keywords: bioelectrochemistry; biophysics; immobilization; membrane proteins; proton transport.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Electrochemical Techniques
  • Electrodes
  • Hydrogen / chemistry*
  • Hydrogen / metabolism
  • Hydrogenase / chemistry
  • Hydrogenase / metabolism
  • Lipid Bilayers / chemistry
  • Microscopy, Atomic Force
  • Mitochondrial Proton-Translocating ATPases / chemistry
  • Mitochondrial Proton-Translocating ATPases / metabolism
  • Oxidation-Reduction
  • Quartz Crystal Microbalance Techniques

Substances

  • Lipid Bilayers
  • Hydrogen
  • Adenosine Triphosphate
  • nickel-iron-selenium hydrogenase
  • Hydrogenase
  • F1F0-ATP synthase
  • Mitochondrial Proton-Translocating ATPases