Whole genome sequence of the emerging oomycete pathogen Pythium insidiosum strain CDC-B5653 isolated from an infected human in the USA

Marina S Ascunce; Jose C Huguet-Tapia; Edward L Braun; Almudena Ortiz-Urquiza; Nemat O Keyhani; Erica M Goss

doi:10.1016/j.gdata.2015.11.019

Whole genome sequence of the emerging oomycete pathogen Pythium insidiosum strain CDC-B5653 isolated from an infected human in the USA

Genom Data. 2015 Nov 23:7:60-1. doi: 10.1016/j.gdata.2015.11.019. eCollection 2016 Mar.

Authors

Marina S Ascunce¹, Jose C Huguet-Tapia², Edward L Braun³, Almudena Ortiz-Urquiza⁴, Nemat O Keyhani⁴, Erica M Goss¹

Affiliations

¹ Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States; Department of Plant Pathology, University of Florida, Gainesville, FL, United States.
² Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States.
³ Department of Biology and Genetics Institute, University of Florida, Gainesville, FL, United States.
⁴ Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, United States.

Abstract

Pythium insidiosum ATCC 200269 strain CDC-B5653, an isolate from necrotizing lesions on the mouth and eye of a 2-year-old boy in Memphis, Tennessee, USA, was sequenced using a combination of Illumina MiSeq (300 bp paired-end, 14 millions reads) and PacBio (10 Kb fragment library, 356,001 reads). The sequencing data were assembled using SPAdes version 3.1.0, yielding a total genome size of 45.6 Mb contained in 8992 contigs, N50 of 13 Kb, 57% G + C content, and 17,867 putative protein-coding genes. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession JRHR00000000.

Keywords: Genome sequencing; Human emerging pathogen; Oomycete; Pythiosis; Pythium insidiosum.