Expression analysis of ClpB/Hsp100 gene in faba bean (Vicia faba L.) plants in response to heat stress

Ritesh Kumar; Amit Kumar Singh; Dhruv Lavania; Manzer H Siddiqui; Mohamed H Al-Whaibi; Anil Grover

doi:10.1016/j.sjbs.2015.03.006

Expression analysis of ClpB/Hsp100 gene in faba bean (Vicia faba L.) plants in response to heat stress

Saudi J Biol Sci. 2016 Mar;23(2):243-7. doi: 10.1016/j.sjbs.2015.03.006. Epub 2015 Mar 24.

Authors

Ritesh Kumar¹, Amit Kumar Singh¹, Dhruv Lavania¹, Manzer H Siddiqui², Mohamed H Al-Whaibi², Anil Grover¹

Affiliations

¹ Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi 110021, India.
² Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia.

Abstract

Heat stress adversely affects the growth and yield of faba bean crop. Accumulation of ClpB/Hsp100 class of proteins is a critical parameter in induction of acquired heat stress tolerance in plants. Heat-induced expression of ClpB/Hsp100 genes has been noted in diverse plant species. Using primers complementary to soybean ClpB/Hsp100 gene, we analyzed the transcript expression profile of faba bean ClpB/Hsp100 gene in leaves of seedlings and flowering plants and in pollen grains. ClpB/Hsp100 protein accumulation profile was analyzed in leaves of faba bean seedlings using Arabidopsis thaliana cytoplasmic Hsp101 antibodies. The transcript and protein levels of faba bean ClpB/Hsp100 were significantly induced in response to heat stress.

Keywords: ClpB; Faba bean; Heat stress; Hsp100; Pollen; Protein expression; Thermotolerance; Transcript induction.