Lactobacillus fermentum Suo Attenuates HCl/Ethanol Induced Gastric Injury in Mice through Its Antioxidant Effects

Huayi Suo; Xin Zhao; Yu Qian; Peng Sun; Kai Zhu; Jian Li; Baozhong Sun

doi:10.3390/nu8030155

Lactobacillus fermentum Suo Attenuates HCl/Ethanol Induced Gastric Injury in Mice through Its Antioxidant Effects

Nutrients. 2016 Mar 10;8(3):155. doi: 10.3390/nu8030155.

Authors

Huayi Suo^{1

2}, Xin Zhao^{3

4

5}, Yu Qian^{6

7

8}, Peng Sun^{9

10

11}, Kai Zhu^{12

13

14}, Jian Li¹⁵, Baozhong Sun¹⁶

Affiliations

¹ Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China. birget@swu.edu.cn.
² College of Food Science, Southwest University, Chongqing 400715, China. birget@swu.edu.cn.
³ Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China. zhaoxin@cque.edu.cn.
⁴ Chongqing Collaborative Innovation Center of Functional Food, Chongqing University of Education, Chongqing 400067, China. zhaoxin@cque.edu.cn.
⁵ Chongqing Engineering Technology Research Center for Functional Food, Chongqing University of Education, Chongqing 400067, China. zhaoxin@cque.edu.cn.
⁶ Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China. qianyu@foods.ac.cn.
⁷ Chongqing Collaborative Innovation Center of Functional Food, Chongqing University of Education, Chongqing 400067, China. qianyu@foods.ac.cn.
⁸ Chongqing Engineering Technology Research Center for Functional Food, Chongqing University of Education, Chongqing 400067, China. qianyu@foods.ac.cn.
⁹ Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China. sunpeng@foods.ac.cn.
¹⁰ Chongqing Collaborative Innovation Center of Functional Food, Chongqing University of Education, Chongqing 400067, China. sunpeng@foods.ac.cn.
¹¹ Chongqing Engineering Technology Research Center for Functional Food, Chongqing University of Education, Chongqing 400067, China. sunpeng@foods.ac.cn.
¹² Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China. zhukai@foods.ac.cn.
¹³ Chongqing Collaborative Innovation Center of Functional Food, Chongqing University of Education, Chongqing 400067, China. zhukai@foods.ac.cn.
¹⁴ Chongqing Engineering Technology Research Center for Functional Food, Chongqing University of Education, Chongqing 400067, China. zhukai@foods.ac.cn.
¹⁵ Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, China. baozhongsun@163.com.
¹⁶ Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China. lijian@swun.cn.

Abstract

The purpose of the study was to determine the inhibitory effects of Lactobacillus fermentum Suo (LF-Suo) on HCl/ethanol induced gastric injury in ICR (Institute for Cancer Research) mice and explain the mechanism of these effects through the molecular biology activities of LF-Suo. The studied mice were divided into four groups: healthy, injured, LF-Suo-L and LF-Suo-H group. After the LF-Suo intragastric administration, the gastric injury area was reduced compared to the injured group. The serum MOT (motilin), SP (substance P), ET (endothelin) levels of LF-Suo treated mice were lower, and SS (somatostatin), VIP (vasoactive intestinal peptide) levels were higher than the injured group mice. The cytokine IL-6 (interleukin 6), IL-12 (interleukin 12), TNF-α (tumor necrosis factor-α) and IFN-γ (interferon-γ) serum levels were decreased after the LF-Suo treatment. The gastric tissues SOD (superoxide dismutase), GSH-Px (glutathione peroxidase), NO (nitric oxide) and activities of LF-Suo treated mice were increased and MDA (malondialdehyde) activity was decreased compared to the injured group mice. By the RT-PCR assay, LF-Suo raised the occludin, EGF (epidermal growth factor), EGFR (epidermal growth factor receptor), VEGF (vascular endothelial growth factor), Fit-1 (fms-like tyrosine kinase-1), IκB-α (inhibitor kappaB-α), nNOS (neuronal nitric oxide synthase), eNOS (endothelial nitric oxide synthase), Mn-SOD, Cu/Zn-SOD, CAT (catalase) mRNA or protein expressions and reduced the COX-2, NF-κB (nuclear factor kappaB), and iNOS (inducible nitric oxide synthase) expressions in gastric tissues compared to the gastric injured group mice. A high concentration (1.0 × 10⁸ CFU/kg b.w.) of LF-Suo treatment showed stronger anti-gastric injury effects compared to a low concentration of (0.5 × 10⁸ CFU/kg b.w.) of LF-Suo treatment. LF-Suo also showed strong survival in pH 3.0 man-made gastric juice and hydrophobic properties. These results indicate that LF-Suo has potential use as probiotics for its gastric injury treatment effects.

Keywords: Lactobacillus fermentum Suo; antioxidant; ethanol; gastric injury; mice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antioxidants / metabolism*
Cytokines / metabolism
Disease Models, Animal
Ethanol*
Gastric Juice / metabolism
Gastric Mucosa / metabolism
Gastrointestinal Hormones / metabolism
Gene Expression Regulation, Enzymologic
Hydrochloric Acid*
Hydrogen-Ion Concentration
Inflammation Mediators / metabolism
Limosilactobacillus fermentum / physiology*
Male
Mice, Inbred ICR
Probiotics*
Stomach / microbiology*
Stomach Ulcer / chemically induced
Stomach Ulcer / microbiology
Stomach Ulcer / pathology
Stomach Ulcer / prevention & control*

Substances

Antioxidants
Cytokines
Gastrointestinal Hormones
Inflammation Mediators
Ethanol
Hydrochloric Acid