Procedure optimization for extracting short-chain fatty acids from human faeces

Justyna Dobrowolska Iwanek; Paweł Zagrodzki; Michał Woźniakiewicz; Aneta Woźniakiewicz; Małgorzata Zwolińska Wcisło; Diana Winnicka; Paweł Paśko

doi:10.1016/j.jpba.2016.02.042

Procedure optimization for extracting short-chain fatty acids from human faeces

J Pharm Biomed Anal. 2016 May 30:124:337-340. doi: 10.1016/j.jpba.2016.02.042. Epub 2016 Mar 3.

Authors

Justyna Dobrowolska Iwanek¹, Paweł Zagrodzki², Michał Woźniakiewicz³, Aneta Woźniakiewicz³, Małgorzata Zwolińska Wcisło⁴, Diana Winnicka¹, Paweł Paśko⁵

Affiliations

¹ Department of Food Chemistry and Nutrition, Medical College Jagiellonian University, 30-688 Kraków, Medyczna 9, Poland.
² Department of Food Chemistry and Nutrition, Medical College Jagiellonian University, 30-688 Kraków, Medyczna 9, Poland; Department of Nuclear Physical Chemistry, Institute of Nuclear Physics, Polish Academy of Scienece, 31-142 Kraków, Radzikowskiego 152, Poland.
³ Laboratory for Forensic Chemistry, Faculty of Chemistry, Jagiellonian University in Kraków, 30-060, Ingardena 3, Poland.
⁴ Department of Gastroenterology, Hepatology and Infectious Diseases, Medical College Jagiellonian University, 31-501 Kraków, Śniadeckich 5, Poland.
⁵ Department of Food Chemistry and Nutrition, Medical College Jagiellonian University, 30-688 Kraków, Medyczna 9, Poland; Faculty of Health and Medical Science, Andrzej Frycz Modrzewski Kraków University, 30-705, Herlinga-Grudzińskiego 1, Poland. Electronic address: paskopaw@poczta.fm.

PMID: 26977586
DOI: 10.1016/j.jpba.2016.02.042

Abstract

Short-chain fatty acids play an important role in the physiology and metabolism of the colon. Disturbed balance of such compounds in human gut can significantly contribute to etiological factors of various gastrointestinal disorders and it may also increase the risk of developing cancer or cardiovascular diseases. The aim of the study was to select the optimal parameters for acetic, propionic and butyric acids extraction from stool samples. The experimental conditions were optimized with respect to the solvent sample shacking time, sample ultrasounds (Ultrasound-Assisted Extraction, UAE) exposure time and the number of extractions from the particulate stool samples. The screening of experimental parameters was conducted with fractional factorial design of experiments, namely 3(3-1). The optimal conditions for UAE were found, namely ultrasound digestion time of 40 min (at 35°C), shaking time of 4 min, and the three subsequent extractions.

Keywords: Extraction; Optimization; Short-chain fatty acids; Stool.

MeSH terms

Adult
Fatty Acids, Volatile / isolation & purification*
Feces / chemistry*
Humans
Male

Substances

Fatty Acids, Volatile