Second harmonic generation double stokes Mueller polarimetric microscopy of myofilaments

Lukas Kontenis; Masood Samim; Abiramy Karunendiran; Serguei Krouglov; Bryan Stewart; Virginijus Barzda

doi:10.1364/BOE.7.000559

Second harmonic generation double stokes Mueller polarimetric microscopy of myofilaments

Biomed Opt Express. 2016 Jan 19;7(2):559-69. doi: 10.1364/BOE.7.000559. eCollection 2016 Feb 1.

Authors

Lukas Kontenis¹, Masood Samim², Abiramy Karunendiran³, Serguei Krouglov¹, Bryan Stewart³, Virginijus Barzda¹

Affiliations

¹ Department of Physics and Institute for Optical Sciences, University of Toronto, 60 St. George Street, Toronto, ON, M5S 1A7, Canada; Department of Chemical and Physical Sciences, University of Toronto, 3359 Mississauga Road, Mississauga, ON, L5L 1C6, Canada.
² Department of Physics and Institute for Optical Sciences, University of Toronto, 60 St. George Street, Toronto, ON, M5S 1A7, Canada; Department of Chemical and Physical Sciences, University of Toronto, 3359 Mississauga Road, Mississauga, ON, L5L 1C6, Canada; Princess Margaret Cancer Center/University Health Network, 610 University Avenue, Toronto, ON, M5G 2M3, Canada.
³ Department of Biology, University of Toronto, 3359 Mississauga Road, Mississauga, ON, L5L 1C6, Canada; Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, ON, M5S 3G5, Canada.

Abstract

The experimental implementation of double Stokes Mueller polarimetric microscopy is presented. This technique enables a model-independent and complete polarimetric characterization of second harmonic generating samples using 36 Stokes parameter measurements at different combinations of incoming and outgoing polarizations. The degree of second harmonic polarization and the molecular nonlinear susceptibility ratio are extracted for individual focal volumes of a fruit fly larva wall muscle.

Keywords: (110.5405) Polarimetric imaging; (170.3880) Medical and biological imaging; (180.4315) Nonlinear microscopy.