Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use

Florian Petry; J Robert Smith; Jasmin Leber; Denise Salzig; Peter Czermak; Mark L Weiss

doi:10.1155/2016/4834616

Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use

Stem Cells Int. 2016:2016:4834616. doi: 10.1155/2016/4834616. Epub 2016 Feb 8.

Authors

Florian Petry¹, J Robert Smith², Jasmin Leber³, Denise Salzig³, Peter Czermak⁴, Mark L Weiss²

Affiliations

¹ Department of Anatomy and Physiology, Kansas State University, 228 Coles Hall, Manhattan, KS 66506, USA; Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstraße 14, 35390 Gießen, Germany.
² Department of Anatomy and Physiology, Kansas State University, 228 Coles Hall, Manhattan, KS 66506, USA.
³ Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstraße 14, 35390 Gießen, Germany.
⁴ Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstraße 14, 35390 Gießen, Germany; Department of Chemical Engineering, Kansas State University, 1005 Durland Hall, Manhattan, KS 66506, USA; Faculty of Biology and Chemistry, Justus Liebig University, Ludwigstraße 23, 35390 Gießen, Germany; Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Project Group Bioresources, Winchester Straße 2, 35392 Gießen, Germany.

Abstract

The great properties of human mesenchymal stromal cells (hMSCs) make these cells an important tool in regenerative medicine. Because of the limitations of hMSCs derived from the bone marrow during isolation and expansion, hMSCs derived from the umbilical cord stroma are a great alternative to overcome these issues. For a large expansion of these cells, we performed a process transfer from static culture to a dynamic system. For this reason, a microcarrier selection out of five microcarrier types was made to achieve a suitable growth surface for the cells. The growth characteristics and metabolite consumption and production were used to compare the cells growth in 12-well plate and spinner flask. The goal to determine relevant process parameters to transfer the expansion process into a stirred tank bioreactor was achieved.