New Labdane diterpenes from Hedychium yunnanense with cytotoxicity and inhibitory effects on nitric oxide production

Yu-Peng Li; Si-Meng Zhao; Jun-Ju Xu; Guang-Zhi Zeng; Yan Li; Ning-Hua Tan; Ye Yang

doi:10.1080/14786419.2016.1143829

New Labdane diterpenes from Hedychium yunnanense with cytotoxicity and inhibitory effects on nitric oxide production

Nat Prod Res. 2016 Dec;30(23):2669-2674. doi: 10.1080/14786419.2016.1143829. Epub 2016 Mar 11.

Authors

Yu-Peng Li^{1

2}, Si-Meng Zhao³, Jun-Ju Xu¹, Guang-Zhi Zeng³, Yan Li³, Ning-Hua Tan³, Ye Yang⁴

Affiliations

¹ a Yunnan Research Center on Good Agricultural Practice for Dominant Chinese Medicinal Materials , Yunnan Agricultural University , Kunming , P.R. China.
² b School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products , Kunming Medical University , Kunming , P.R. China.
³ c State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany , Chinese Academy of Sciences , Kunming , P.R. China.
⁴ d Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming , P.R. China.

PMID: 26965832
DOI: 10.1080/14786419.2016.1143829

Abstract

Two new labdane diterpenes, hedychenoids A (1) and B (2), were isolated from the rhizomes of Hedychium yunnanense, together with four known ones hedychenone (3), forrestin A (4), villosin (5) and calcaratarin C (6). Their structures were determined on the basis of NMR (1D and 2D) and mass spectroscopic analysis. Compounds 2, 3 and 5 exhibited cytotoxicity against SGC-7901 with IC₅₀ values of 14.88 ± 0.52, 7.08 ± 0.21 and 7.76 ± 0.21 μg/ml, 3 and 5 against HeLa with IC₅₀ values of 9.76 ± 0.48 and 13.24 ± 0.63 μg/ml, respectively. Compounds 2, 5 showed inhibitory effects against nitric oxide production in LPS and IFN-γ-induced RAW 264.7 murine macrophages with IC₅₀ values of 6.57 ± 0.88 and 5.99 ± 1.20 μg/ml, respectively.

Keywords: Zingiberaceae; cytotoxicity; inhibition of NO production; labdane diterpenes.