Lipoxygenase inhibitors from the latex of Calotropis Procera

Wael M Abdel-Mageed; Nadia H Mohamed; Miaomiao Liu; Ali A El-Gamal; Omer A Basudan; Mady Ahmed Ismail; Ronald J Quinn; Xueting Liu; Lixin Zhang; Ahmed A M Shoreit

doi:10.1007/s12272-016-0725-9

Lipoxygenase inhibitors from the latex of Calotropis Procera

Arch Pharm Res. 2016 Mar 9. doi: 10.1007/s12272-016-0725-9. Online ahead of print.

Authors

Wael M Abdel-Mageed^{1

2}, Nadia H Mohamed^{3

4}, Miaomiao Liu^{5

6}, Ali A El-Gamal¹, Omer A Basudan¹, Mady Ahmed Ismail³, Ronald J Quinn⁷, Xueting Liu⁸, Lixin Zhang⁹, Ahmed A M Shoreit¹⁰

Affiliations

¹ Pharmacognosy Department, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia.
² Pharmacognosy Department, Faculty of Pharmacy, Assiut University, Assiut, 71526, Egypt.
³ Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, Egypt.
⁴ Department of Biology, Faculty of Science and Art, Samtah, Jazan University, Jazan, Saudi Arabia.
⁵ Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
⁶ Graduate University of Chinese Academy of Sciences, Beijing, 100049, China.
⁷ Eskitis Institute, Griffith University, Brisbane, QLD, 4111, Australia.
⁸ Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. liuxueting_cn@hotmail.com.
⁹ Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. zhanglixin@im.ac.cn.
¹⁰ Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, Egypt. ashoreit1968@yahoo.com.

PMID: 26960736
DOI: 10.1007/s12272-016-0725-9

Abstract

A radical-scavenging, guided phytochemical study of the latex of Calotropis Procera afforded five lignans (1-5), including a new one (4). The structural determination was accomplished using 1D- and 2D-NMR, high-resolution electrospray ionization mass spectrometry (HRESIMS), and correlation with known compounds. Among the isolated compounds, acylated lignans (3-5) showed stronger antioxidant activity than non-acylated derivatives (1,2). Anti-inflammatory activity was evaluated by determining the inhibitory potential against 5- and 15-lipoxygenase enzymes. The highest anti-inflammatory activity was observed in compound 4, with IC_50s values of 7.6 µM and 2.7 µM against 5-LOX and 15-LOX, respectively.

Keywords: Anti-inflammatory; Antioxidants; Calotropis procera; Lignans; Lipoxygenase inhibitors.