Abstract
PS B, a naturally occurring CD4(+) T-cell simulating zwitterionic polysaccharide from Bacteroides fragilis ATCC 25285/NCTC 9343, was conjugated with aminooxy Thomsen Friedenreich (TF or T) [α-d-Gal-(1,3)-β-d-GalNAc-ONH2] tumor antigen. Immunization in Jax C57BL/6, followed by ELISA revealed IgM and IgG antibody TF specificity. FACS data noted preferential binding to TF-laced MCF-7 cells but not to HCT-116 cells.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Antigen-Antibody Reactions
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Antigens, Tumor-Associated, Carbohydrate / chemistry
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Antigens, Tumor-Associated, Carbohydrate / immunology*
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Bacteroides fragilis / chemistry
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Carbohydrate Conformation
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Enzyme-Linked Immunosorbent Assay
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HCT116 Cells
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Humans
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Immunoglobulin G / chemistry
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Immunoglobulin G / immunology
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Immunoglobulin M / chemistry
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Immunoglobulin M / immunology
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MCF-7 Cells
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Mice
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Mice, Inbred C57BL
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Polysaccharides / chemistry
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Polysaccharides / immunology*
Substances
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Antigens, Tumor-Associated, Carbohydrate
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Immunoglobulin G
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Immunoglobulin M
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Polysaccharides
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Thomsen-Friedenreich antigen