Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization

Molly E Church; Florante N Dela Cruz; Kevin Kim; Michele Persiani; Leslie W Woods; Patricia A Pesavento; Kevin D Woolard

doi:10.1016/j.dib.2016.01.070

Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization

Data Brief. 2016 Feb 9:7:60-5. doi: 10.1016/j.dib.2016.01.070. eCollection 2016 Jun.

Authors

Molly E Church¹, Florante N Dela Cruz¹, Kevin Kim², Michele Persiani³, Leslie W Woods³, Patricia A Pesavento¹, Kevin D Woolard¹

Affiliations

¹ UC Davis, School of Veterinary Medicine, Department of Pathology, Microbiology, and Immunology, USA.
² UC Davis, School of Medicine, Department of Dermatology, USA.
³ UC Davis, School of Veterinary Medicine, California Animal Health and Food Safety Laboratory System, USA.

Abstract

Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.