Analysis of enzyme-responsive peptide surfaces by Raman spectroscopy

Jugal Kishore Sahoo; Narayana M S Sirimuthu; Anne Canning; Mischa Zelzer; Duncan Graham; Rein V Ulijn

doi:10.1039/c5cc09189f

Analysis of enzyme-responsive peptide surfaces by Raman spectroscopy

Chem Commun (Camb). 2016 Mar 28;52(25):4698-701. doi: 10.1039/c5cc09189f.

Authors

Jugal Kishore Sahoo¹, Narayana M S Sirimuthu², Anne Canning³, Mischa Zelzer⁴, Duncan Graham⁵, Rein V Ulijn⁶

Affiliations

¹ WestCHEM, Department of Pure & Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, 99 George Street, Glasgow, G1 1RD, UK.
² Centre of Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, 99 George Street, Glasgow, G1 1RD, UK. Duncan.graham@strath.ac.uk and Department of Chemistry, University of Sri Jayewardenepura, Sri Lanka.
³ University of Nottingham, School of Pharmacy, University Park, Boots Science Building, Nottingham, NG7 2RD, UK.
⁴ University of Nottingham, School of Pharmacy, University Park, Boots Science Building, Nottingham, NG7 2RD, UK and National Physical Laboratory, Hampton Rd, Teddington, Middlesex TW11 0LW, UK.
⁵ Centre of Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, 99 George Street, Glasgow, G1 1RD, UK. Duncan.graham@strath.ac.uk.
⁶ WestCHEM, Department of Pure & Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, 99 George Street, Glasgow, G1 1RD, UK and Advanced Science Research Center (ASRC) and Hunter College, City University of New York, NY 10031, NY, USA. Rein.Ulijn@asrc.cuny.edu.

Abstract

We report on the use of Raman spectroscopy as a tool to characterise model peptide functionalised surfaces. By taking advantage of Raman reporters built into the peptide sequence, the enzymatic hydrolysis of these peptides could be determined.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Hydrolysis
Models, Theoretical
Pancreas / enzymology
Pancreatic Elastase / chemistry
Pancreatic Elastase / metabolism*
Peptides / chemical synthesis
Peptides / chemistry
Peptides / metabolism*
Spectrum Analysis, Raman*
Surface Properties
Swine

Substances

Peptides
Pancreatic Elastase