Development of a fast DNA extraction method for sea food and marine species identification

Marcello Tagliavia; Aldo Nicosia; Monica Salamone; Girolama Biondo; Carmelo Daniele Bennici; Salvatore Mazzola; Angela Cuttitta

doi:10.1016/j.foodchem.2016.02.095

Development of a fast DNA extraction method for sea food and marine species identification

Food Chem. 2016 Jul 15:203:375-378. doi: 10.1016/j.foodchem.2016.02.095. Epub 2016 Feb 15.

Authors

Marcello Tagliavia¹, Aldo Nicosia², Monica Salamone³, Girolama Biondo⁴, Carmelo Daniele Bennici⁵, Salvatore Mazzola⁶, Angela Cuttitta⁷

Affiliations

¹ National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: marcello.tagliavia@iamc.cnr.it.
² National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: aldo.nicosia@iamc.cnr.it.
³ National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: monica.salamone@iamc.cnr.it.
⁴ National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: girolama.biondo@iamc.cnr.it.
⁵ National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: carmelo.bennici@iamc.cnr.it.
⁶ National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: salvatore.mazzola@iamc.cnr.it.
⁷ National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: angela.cuttitta@iamc.cnr.it.

PMID: 26948627
DOI: 10.1016/j.foodchem.2016.02.095

Abstract

The authentication of food components is one of the key issues in food safety. Similarly taxonomy, population and conservation genetics as well as food web structure analysis, also rely on genetic analyses including the DNA barcoding technology. In this scenario we developed a fast DNA extraction method without any purification step from fresh and processed seafood, suitable for any PCR analysis. The protocol allows the fast DNA amplification from any sample, including fresh, stored and processed seafood and from any waste of industrial fish processing, independently of the sample storage method. Therefore, this procedure is particularly suitable for the fast processing of samples and to carry out investigations for the authentication of seafood by means of DNA analysis.

Keywords: DNA barcoding; DNA extraction; Marine species; Mislabeled seafood; PCR amplification; Species identification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA / analysis*
DNA / isolation & purification
DNA Barcoding, Taxonomic / methods*
Fishes / classification*
Fishes / genetics*
Seafood / analysis
Seafood / classification*

Substances

DNA