Development of pan-phlebovirus RT-PCR assay

Alexander S Klimentov; Alexander M Butenko; Natalia V Khutoretskaya; Elena Yu Shustova; Victor F Larichev; Olga V Isaeva; Galina G Karganova; Alexander N Lukashev; Anatoly P Gmyl

doi:10.1016/j.jviromet.2016.02.009

Development of pan-phlebovirus RT-PCR assay

J Virol Methods. 2016 Jun:232:29-32. doi: 10.1016/j.jviromet.2016.02.009. Epub 2016 Mar 4.

Authors

Alexander S Klimentov¹, Alexander M Butenko², Natalia V Khutoretskaya², Elena Yu Shustova³, Victor F Larichev², Olga V Isaeva³, Galina G Karganova⁴, Alexander N Lukashev³, Anatoly P Gmyl⁵

Affiliations

¹ Chumakov Institute of Poliomyelitis and Viral Encephalitides, Kievskoe shosse 27km., Moscow 142782, Russia; D.I. Ivanovsky Institute of Virology of N.F. Gamaleya Center of Epidemiology and Microbiology, Gamaleya Str. 16, Moscow 123098, Russia.
² D.I. Ivanovsky Institute of Virology of N.F. Gamaleya Center of Epidemiology and Microbiology, Gamaleya Str. 16, Moscow 123098, Russia.
³ Chumakov Institute of Poliomyelitis and Viral Encephalitides, Kievskoe shosse 27km., Moscow 142782, Russia.
⁴ Chumakov Institute of Poliomyelitis and Viral Encephalitides, Kievskoe shosse 27km., Moscow 142782, Russia; M.V. Lomonosov Moscow State University, Department of Biology, Leninskiye Gory Str. 1, Moscow 119991, Russia.
⁵ Chumakov Institute of Poliomyelitis and Viral Encephalitides, Kievskoe shosse 27km., Moscow 142782, Russia. Electronic address: apgmyl@mail.ru.

PMID: 26947398
DOI: 10.1016/j.jviromet.2016.02.009

Abstract

This study reports the pan-phlebovirus assay capable of detecting both sandfly/mosquito- and tick-borne phleboviruses. Sensitivity and specificity of the assay was verified using a panel of arboviruses. The RT-PCR assay is simple and sensitive, and thus well suited for screening of field samples.

Keywords: Phlebovirus; RT-PCR assay; Sandfly/mosquito-borne; Tick-borne.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bunyaviridae Infections / diagnosis*
Bunyaviridae Infections / virology
Humans
Molecular Diagnostic Techniques / methods*
Phlebovirus / classification*
Phlebovirus / genetics
Phlebovirus / isolation & purification*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Virology / methods*