In vivo expression of genes in the entomopathogenic fungus Beauveria bassiana during infection of lepidopteran larvae

Sandhya Galidevara; Annette Reineke; Uma Devi Koduru

doi:10.1016/j.jip.2016.03.002

In vivo expression of genes in the entomopathogenic fungus Beauveria bassiana during infection of lepidopteran larvae

J Invertebr Pathol. 2016 May:136:32-4. doi: 10.1016/j.jip.2016.03.002. Epub 2016 Mar 2.

Authors

Sandhya Galidevara¹, Annette Reineke², Uma Devi Koduru³

Affiliations

¹ Department of Botany, Andhra University, Visakhapatnam, 530003 AP, India.
² Hochschule Geisenheim University, Department of Phytomedicine, Von-Lade-Str. 1, D-65366 Geisenheim, Germany.
³ Department of Botany, Andhra University, Visakhapatnam, 530003 AP, India. Electronic address: umadevikoduru@gmail.com.

PMID: 26945772
DOI: 10.1016/j.jip.2016.03.002

Abstract

The entomopathogenic fungus Beauveria bassiana (Bals.) Vuillemin is commercially available as a bio insecticide. The expression of three genes previously identified to have a role in pathogenicity in in vitro studies was validated in vivo in three lepidopteran insects infected with B. bassiana. Expression of all three genes was observed in all the tested insects starting from 48 or 72h to 10d post infection corroborating their role in pathogenicity. We suggest that it is essential to test the expression of putative pathogenicity genes both in vitro and in vivo to understand their role in different insect species.

Keywords: Beauveria bassiana; Entomopathogenic fungus; In vivo expression; Pathogenicity genes; qPCR.

MeSH terms

Animals
Beauveria / genetics*
Beauveria / pathogenicity*
Gene Expression Profiling
Gene Expression Regulation, Fungal
Genes, Fungal / genetics*
Moths / microbiology*
Mycoses / genetics
Real-Time Polymerase Chain Reaction
Transcriptome